FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-84-5-7-5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-84-5-7-5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19480
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT589330.25154004106776No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT18249.363449691991786No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCG14757.571868583162218No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC7333.7628336755646816No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTCT6843.5112936344969197No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT5662.9055441478439423No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTTC3461.7761806981519506No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT3351.7197125256673513No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTGT2661.3655030800821355No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT2341.2012320328542094No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCG2331.1960985626283367No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCG2311.1858316221765914No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGCT2271.1652977412731005No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCTG1930.9907597535934293No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTTC1870.9599589322381931No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1840.944558521560575No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC1820.9342915811088296No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTCT1650.8470225872689939No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1540.7905544147843943No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTCT1440.7392197125256673No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCGC1420.728952772073922No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1410.7238193018480493No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC1400.7186858316221766No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTTC1000.5133470225872689No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC970.49794661190965095No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT960.49281314168377827No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC960.49281314168377827No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTTC860.4414784394250513No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG820.4209445585215606No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT690.3542094455852156No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCGC670.34394250513347024No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT630.32340862422997946No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTCT620.3182751540041068No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTCG620.3182751540041068No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT590.3028747433264887No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCGCTT580.297741273100616No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCGC560.2874743326488706No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTCT540.27720739219712526No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCTT530.2720739219712526No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTCG520.2669404517453799No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCCC510.2618069815195072No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA500.25667351129363447No Hit
ATCTGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG500.25667351129363447No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATCC460.23613963039014374No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAGTC450.23100616016427106No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCGC450.23100616016427106No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTAGGGGTATCTAATCCCAGTC450.23100616016427106No Hit
ATCTGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCGCTTCTG440.22587268993839835No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTCGC440.22587268993839835No Hit
ATCTGTAGAACAGGCTCCTCTAGGGCTACAGGGGTATCTAATCCCAGTCG430.22073921971252566No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG420.215605749486653No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCGCTT420.215605749486653No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCGC410.2104722792607803No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTCG390.2002053388090349No Hit
ATCTGTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCTTC370.18993839835728954No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTCGC360.18480492813141683No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGCTT340.17453798767967144No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTCT340.17453798767967144No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT320.16427104722792607No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT320.16427104722792607No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGTCGGGGTATCTAATCCCAGTCG310.1591375770020534No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCG310.1591375770020534No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAGTCG310.1591375770020534No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT310.1591375770020534No Hit
ATCTGTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTCGCTTC290.148870636550308No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTCTGGGCTCGGAGGGGTATCTAATC280.1437371663244353No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCGCT270.13860369609856263No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT260.13347022587268995No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAATC260.13347022587268995No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT260.13347022587268995No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT260.13347022587268995No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT250.12833675564681724No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGTGGGGTATCTAATCCCAGTCGCT230.11806981519507187No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT220.11293634496919917No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCGCTT220.11293634496919917No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTCT220.11293634496919917No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCGC210.1078028747433265No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGTGGGGGTATCTAATCCCAGTCGC210.1078028747433265No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTCG200.10266940451745381No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT200.10266940451745381No Hit
ATCTGTAGAACAGGCTCCTCTAGCACGGGGGTATCTAATCCCAGTCGCTT200.10266940451745381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTA19200.0294.999971
TCTGTAG19250.0294.233762
CTGTAGA19300.0293.47153
GAACAGG19050.0292.677158
AACAGGC19050.0291.902869
GTAGAAC19300.0291.178775
TGTAGAA19500.0289.705144
TAGAACA20100.0279.589546
AGAACAG20200.0276.745067
TGTGAAC150.0028282893196.666664
ACTAAGG150.0028282893196.66666295
CTGTGAA150.0028282893196.666663
ATCTGTG200.0066872104147.51
CAGTTTT6600.059.00000485-89
TATATTT1550.059.00000495-99
GGCACCG2750.059.00000430-34
AGGTGGG2850.059.00000420-24
GTTTAGG8100.059.000004110-114
GCTGTGG7200.059.00000460-64
AGGCTCC19100.059.00000410-14
Produced by FastQC (version 0.11.5)