FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-83-4-7-5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-83-4-7-5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19619
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT385219.634028237932615No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC243012.385952393088333No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT13226.738365869820072No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT12086.157296498292472No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTC6403.262143840154952No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC4072.07451959834854No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3942.008257301595392No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCT3711.8910240073398237No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3501.7839849125847393No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTT3171.6157806208267498No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTT3131.595392221825781No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT3021.539324124573118No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC2761.406799531066823No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA2331.1876242418064122No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCT2291.1672358428054437No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTC2241.1417503440542331No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT2231.1366532443039912No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGC2091.0652938478006013No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG1830.9327692542943066No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1750.8919924562923697No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1680.8563127580406749No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTT1580.8053417605382538No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1570.8002446607880116No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTG1450.7390794637851062No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1260.6422345685305061No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1200.6116519700290535No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTCG1180.6014577705285693No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCT1080.5504867730261481No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCG1080.5504867730261481No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1070.5453896732759059No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCG880.44854477802130593No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCG870.44344767827106374No Hit
GAGACTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC850.4332534787705795No Hit
GAGACTTAGAACAGGCTCCTCTAGCGGCAGGTCAGGGGTATCTAATCCCA840.42815637902033743No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTC830.42305927927009535No Hit
GAGACTTAGAACAGGCTCCTCTAGCACAGGGGTATCTAATCCCAGTCGCT830.42305927927009535No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCG740.3771853815179163No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTC710.36189408226718994No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCGCTT670.34150568326622155No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGT670.34150568326622155No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCG670.34150568326622155No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCG640.3262143840154952No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGTGGGCTCGGGGTATCTAATC630.32111728426525304No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT620.31602018451501096No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCTTC600.30582598501452674No Hit
GAGACTTAGAACAGGCTCCTCTATGCGTCAGGGGTATCTAATCCCAGTCG550.2803404862633162No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG550.2803404862633162No Hit
GAGACTTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTCGCT540.27524338651307406No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTC520.26504918701258984No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTTCT510.2599520872623477No Hit
GAGACTTAGAACAGGCTCCTCTAGGGCTACAGGGGTATCTAATCCCAGTC500.2548549875121056No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC500.2548549875121056No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAA490.24975788776186353No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTCG480.2446607880116214No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT470.23956368826137928No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTT460.23446658851113714No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGCT440.22427238901065297No Hit
GAGACTTAGAACAGGCTCCTCTAGGGCTCGGGGTATCTAATCCCAGTCGC440.22427238901065297No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT430.21917528926041083No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTAAT430.21917528926041083No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT410.20898108975992657No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG400.2038839900096845No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTC390.1987868902594424No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTC390.1987868902594424No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGTCGTATGGGGTATCTAATCCCA380.19368979050920027No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT370.18859269075895815No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTC350.17839849125847393No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCT340.17330139150823182No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA330.1682042917579897No Hit
GAGACTTAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTCGCTT310.15801009225750548No Hit
GAGACTTAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTCGCTT310.15801009225750548No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTC310.15801009225750548No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA300.15291299250726337No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT300.15291299250726337No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTC280.14271879300677914No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCGC270.13762169325653703No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTC270.13762169325653703No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTCG270.13762169325653703No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCGCT270.13762169325653703No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTCACCAGGGGTATCTAATCCCAG260.13252459350629492No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT260.13252459350629492No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC250.1274274937560528No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT210.10703909475508436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT19350.0294.237731
CTTAGAA19350.0292.71325
TTAGAAC19150.0292.689276
GAACAGG19050.0292.677159
AGACTTA19500.0291.974372
ACTTAGA19450.0291.208224
TAGAACA20000.0280.257
AGAACAG20200.0278.205448
GAAAGGC150.0028284553196.666679
TTAGAAA359.817959E-7168.571446
GACTTAG37850.0149.643333
GAAAAGG200.006687603147.59
GCATAAG200.006687603147.5295
GCCCTCG501.8189894E-1259.00000425-29
AGCCTAG150.004368576759.000004120-124
TGCACTC150.004368576759.000004215-219
GAGGCAC1300.059.00000430-34
ACTGCCA452.5465852E-1159.00000435-39
TGTTGAG3750.059.000004105-109
TCCTTGG501.8189894E-1259.00000435-39
Produced by FastQC (version 0.11.5)