FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-82-5-5-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-82-5-5-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19213
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC516126.86202050694842No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG18289.514391297558944No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC16428.546296778223079No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG12436.469577889970332No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATC7383.841149221881018No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG4942.571175766408161No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC4292.232863165564982No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4072.1183573622026755No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3611.8789361369905795No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2691.4000936865663873No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT2611.3584552126164577No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA2371.2335397907666685No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2251.1710820798417738No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA2151.1190339874043616No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC2091.0878051319419144No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCC1810.9420704731171603No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1650.8587935252173008No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATC1510.7859261958049238No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1480.7703117680737002No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCAT1450.7546973403424764No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCAT1400.7286732941237704No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1280.6662155831988757No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1060.551709779836569No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCAT1000.5204809243741216No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT990.5152761151303805No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCA920.47884245042419193No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA910.47363764118045076No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGCGTCAGGGGTATCTAATCCCA890.4632280226929683No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTC840.4372039764742622No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG740.3851558840368501No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT730.37995107479310886No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC720.37474626554936763No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATC710.3695414563056264No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC700.3643366470618852No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC610.31749336386821425No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCA610.31749336386821425No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC600.312288554624473No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCA590.3070837453807318No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA580.30187893613699057No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATC530.2758548899182845No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC530.2758548899182845No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTC460.23942122521209597No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATC450.23421641596835474No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATC430.22380679748087232No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCAGGGGTATCTAATCCCAGTC360.18737313277468381No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCA340.1769635142872014No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC320.16655389579971894No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGT320.16655389579971894No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGAGGGGTATC290.15093946806849529No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCACAGGGGTATCTAATCCCAGT270.14052984958101286No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC260.13532504033727163No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCC240.1249154218497892No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC240.1249154218497892No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGTGGGGTATCTAATCCCAGTC230.11971061260604798No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT230.11971061260604798No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCACGTCGGGGTATCTAATCCCAG230.11971061260604798No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT220.11450580336230677No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTC220.11450580336230677No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGT220.11450580336230677No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG200.10409618487482433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATC18950.0295.000032
GCTACCA108.400433E-4294.99997295
TCAATCT19050.0293.451453
TCTCAAT19200.0292.69531
AATCTAG18900.0292.658755
CAATCTA19100.0291.9114
ATCTAGA19050.0290.354346
CTAGAAC19050.0290.354348
TCTAGAA19050.0289.580087
TAGAACA19650.0280.73799
ATCTAAA352.0909845E-4126.4285666
CATAGTA403.5575754E-4110.62499295
AATCTAA550.001261455680.4545445
CAGAAGA858.0620855E-569.411766295
CTACAGG452.5465852E-1159.00000425-29
TCCTTTG8650.059.00000445-49
ACCGGGG600.059.00000430-34
GGGTTTG452.5465852E-1159.00000425-29
TCCTTAG452.5465852E-1159.00000445-49
GTTTGGG452.5465852E-1159.00000430-34
Produced by FastQC (version 0.11.5)