FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-81-4-7-5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-81-4-7-5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19178
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC340117.733861716550216No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG232812.138909166753571No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT18249.510897903848159No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC10495.469809156324955No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCT8134.239232453853373No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG5172.695797267702576No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG3651.903222442381896No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTC3321.7311502763583273No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3121.626864115131922No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3011.5695067264573992No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2991.5590781103347586No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCT2741.428720408801752No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA2671.3922202523725102No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGC2631.371363020127229No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGC2271.1836479299196996No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT2211.152362081551778No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTT1910.9959328397121702No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC1730.9020752946084055No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1550.8082177495046408No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGC1540.8030034414433205No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCT1520.7925748253206799No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGC1460.7612889769527583No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1440.7508603608301179No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1340.6987172802169153No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1310.6830743560329544No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1220.6361455834810721No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1170.6100740431744707No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1030.537073730315987No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1020.5318594222546668No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG970.5057878819480656No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA920.47971634164146415No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG870.4536448013348629No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG840.4380018771509021No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCTTGTGGGGTATCTAATCCCAG790.41193033684430075No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCT760.39628741266033995No Hit
TCTCAATCTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGT750.3910731045990197No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT710.37021587235373865No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG710.37021587235373865No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT620.32328709980185627No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA590.3076441756178955No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGT590.3076441756178955No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCG570.29721555949525497No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC560.29200125143393474No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC550.28678694337261446No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTT550.28678694337261446No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATC520.27114401918865366No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCG520.27114401918865366No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC520.27114401918865366No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTC510.2659297111273334No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC510.2659297111273334No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG490.25550109500469287No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAG440.22942955469809154No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCT440.22942955469809154No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGCTCTTTCGATCTCGGGGTATCT430.2242152466367713No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCG420.21900093857545105No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGGTATCTAA400.20857232245281052No Hit
TCTCAATCTAGAACAGGCTCCTCTGGACATCGGGGTATCTAATCCCAGTC400.20857232245281052No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTCTCGTGGGGTATCTAATCCCAG400.20857232245281052No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC370.19292939826884972No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC370.19292939826884972No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTCG350.18250078214620918No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA330.17207216602356867No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCT330.17207216602356867No Hit
TCTCAATCTAGAACAGGCTCCTCTCGTCGGTATCTAATCCCAGTCGCTTC330.17207216602356867No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCG320.16685785796224842No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCG310.16164354990092814No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG310.16164354990092814No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGAGGTATCTAAT300.15642924183960788No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC290.15121493377828762No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCT270.1407863176556471No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGTTGGGGTATCTAATCCCAG270.1407863176556471No Hit
TCTCAATCTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTCGCT270.1407863176556471No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCA260.13557200959432683No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCT260.13557200959432683No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGCATCAGGGGTATCTAATCCC250.13035770153300658No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA250.13035770153300658No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT230.11992908541036604No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGGTATCTAATCCCAGTCGC230.11992908541036604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAG18500.0295.05
TCAATCT18700.0295.03
CTCAATC18850.0294.21752
CAATCTA18600.0294.2074
TCTCAAT19100.0292.683231
TCTAGAA18600.0291.827947
ATCTAGA18700.0291.056156
CTAGAAC18650.0291.045568
TAGAACA19500.0278.358989
CTCAATT150.002827949196.666662
CAATCTG255.4720564E-5177.04
CAATTAG200.0066864076147.54
TCAATTA200.0066864076147.53
TCTGAAC352.0909002E-4126.4285667
ATCTGAA352.0909002E-4126.4285666
AATCTGA508.63869E-488.55
TGAACAG508.63869E-488.59
CTGAACA600.001781741473.749998
TTTCTAT1250.059.000004100-104
GTTCCCT1250.059.00000475-79
Produced by FastQC (version 0.11.5)