FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-80-4-1-3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-80-4-1-3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19310
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC334517.322630761263593No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT18219.430346970481615No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATC16748.669083376488866No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC11175.784567581563956No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCG9394.862765406525116No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT6553.392024857586742No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGAT4212.180217503883998No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACC3791.9627136198860695No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACC3761.9471776281719317No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3511.817711030554117No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG3201.657172449508027No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3071.5898498187467631No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2871.4862765406525116No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCG2771.4344899016053858No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT2151.1134127395132056No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTA1991.0305541170378043No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG1971.0201967892283792No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC1830.947695494562403No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1620.8389435525634386No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1530.7923355774210253No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGA1520.7871569135163128No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1330.6887622993267737No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAA1300.6732263076126359No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT1260.6525116519937856No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1080.5592957017089591No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1070.5541170378042465No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC970.5023303987571207No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT940.48679440704298294No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACC920.47643707923355777No Hit
CGATTCCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA860.4453650958052822No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA800.4142931123770067No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTAA760.3935784567581564No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTAA760.3935784567581564No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGA740.3832211289487312No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTA590.30554117037804246No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC580.3003625064733299No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC570.2951838425686173No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCG570.2951838425686173No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAG560.29000517866390474No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGT560.29000517866390474No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTA540.2796478508544796No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAAC540.2796478508544796No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT520.2692905230450544No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAAC510.2641118591403418No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAAC510.2641118591403418No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC500.25893319523562924No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGATCGGGGTATCTAAT490.25375453133091663No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAG490.25375453133091663No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTA480.24857586742620405No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT480.24857586742620405No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTAACCG480.24857586742620405No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCG470.24339720352149147No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTCAGGGGTATCTAATCCCAGTA470.24339720352149147No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCG450.2330398757120663No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCCCTCGATCTCCTTGGGGTATCTA440.22786121180735372No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTAAC420.21750388399792853No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAAC420.21750388399792853No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC410.21232522009321594No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCGTGGGGTATCTAATCCCAGTAA390.20196789228379078No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTGCTCGGAGGGGTATCTAATC390.20196789228379078No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAAC390.20196789228379078No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCC390.20196789228379078No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTCCAGGTCAGGGGTATCTAATCCC390.20196789228379078No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACC380.1967892283790782No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTA370.1916105644743656No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTAAC350.18125323666494045No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTA350.18125323666494045No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAAC350.18125323666494045No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGGTATCTAATCCCA340.17607457276022787No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTGGCTGTCTCTTATACACAGGG320.1657172449508027No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT320.1657172449508027No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCTGGGGTATCTAATCCCAGTA310.16053858104609012No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA300.15535991714137753No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCTCTGGGGTATCTAATCCCAGTA290.15018125323666495No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTAAC280.14500258933195237No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTAAC280.14500258933195237No Hit
CGATTCCTAGAACAGGCTCCTCTACAGGGGTATCTAATCCCAGTAACCGA280.14500258933195237No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGTATCTAATCCCAGTAACC250.12946659761781462No Hit
CGATTCCTAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTAACC250.12946659761781462No Hit
CGATTCCTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTAACCG250.12946659761781462No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACCG250.12946659761781462No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAAC250.12946659761781462No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCG250.12946659761781462No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCCGGGGGTATCTAATCCCAGTAA240.12428793371310203No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC230.11910926980838944No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTAACCG230.11910926980838944No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC220.11393060590367686No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGGGTATCTAAT210.10875194199896426No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC210.10875194199896426No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA210.10875194199896426No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT210.10875194199896426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAG18950.0295.04
GATTCCT19050.0295.02
ATTCCTA19000.0295.03
CGATTCT108.4007723E-4295.01
CGATTCC19150.0294.999971
TCCTAGA18950.0294.221625
CCTAGAA18950.0293.443276
CTAGAAC19100.0290.36657
TAGAACA19950.0277.9958
AGAACAG20100.0275.920389
GATTCTA150.0028281203196.666672
ACGAGCA508.639728E-488.5295
CGTTTGT452.5465852E-1159.00000460-64
GGAGATC257.780209E-659.000004200-204
TCCTTAG501.8189894E-1259.00000445-49
AACTGTT4000.059.000004100-104
GCTAGGC1250.059.000004120-124
GGCGCTT1250.059.00000465-69
CTCCTAG257.780209E-659.00000470-74
GTTCTCT452.5465852E-1159.00000475-79
Produced by FastQC (version 0.11.5)