FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-79-5-5-7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-79-5-5-7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18839
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC254413.503901480970326No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT246913.105791177875684No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC16718.86989755294867No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCC8614.570306279526514No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC6623.5139869419820586No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATC6113.2432719358777007No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA4842.569138489304103No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4802.547905939805722No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAG4022.1338712245872924No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG3932.086097988215935No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCA3691.9587026912256489No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT3431.8206911194861723No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3061.6242900366261481No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC2721.4438133658899093No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATC2561.358883167896385No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC2321.231487870906099No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT2261.1996390466585274No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCAT2201.167790222410956No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCAT1931.024470513296884No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1860.9873135516747172No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1730.918307765804979No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCA1620.8599182546844312No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCA1480.7856043314400978No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTC1220.647592759700621No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1020.5414300122087159No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT1000.5308137374595254No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC970.5148893253357396No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT900.47773236371357286No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC900.47773236371357286No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCC860.45649981421519187No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCC850.4511916768405966No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATC840.44588353946600134No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC800.4246509899676203No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT800.4246509899676203No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCC800.4246509899676203No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCC760.40341844046923936No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTCA710.3768777535962631No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCC690.36626147884707255No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA690.36626147884707255No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAGT650.34502892934869156No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCAT640.33972079197409627No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT620.32910451722490575No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC600.3184882424757153No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG570.3025638303519295No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGT520.27602314347895324No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC500.2654068687297627No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTCGCGTCAGGGGTATCTAATCC470.24948245660597695No Hit
CGATTCCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA460.2441743192313817No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTCATC460.2441743192313817No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC460.2441743192313817No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCATC450.23886618185678643No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCA440.23355804448219117No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCAT420.22294176973300067No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC400.21232549498381015No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC390.2070173576092149No Hit
CGATTCCTAGAACAGGCTCCTCTAAGGACTACCGGGGTATCTAATCCCAG390.2070173576092149No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC380.20170922023461968No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTC380.20170922023461968No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC370.19640108286002445No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGT370.19640108286002445No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCAT310.16455225861245287No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATC310.16455225861245287No Hit
CGATTCCTAGAACAGGCTCCTCTAAGTTGGGGTATCTAATCCCAGTCATC310.16455225861245287No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCAT310.16455225861245287No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATC280.14862784648866711No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCCTGGGGTATCTAATCCCAGT250.13270343436488136No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGATTCTTGGGGTATCTAATCCCA250.13270343436488136No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC240.1273952969902861No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGT240.1273952969902861No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC240.1273952969902861No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCAT240.1273952969902861No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG230.12208715961569085No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCAT220.11677902224109558No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC220.11677902224109558No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC210.11147088486650034No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGCGGGGTATCTAATCCCAGTCA210.11147088486650034No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCATC200.10616274749190507No Hit
CGATTCCTAGAACAGGCTCCTCTAGACCATTTCGGGGGGTATCTAATCCC190.10085461011730984No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAGT190.10085461011730984No Hit
CGATTCCTAGAACAGGCTCCTCTTATAGGGTATCTAATCCCAGTCATCCA190.10085461011730984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA18500.0295.05
CCTAAAC108.399036E-4295.06
TTCCTGA108.399036E-4295.04
TTCCTAG18500.0295.04
ATTCCTG108.399036E-4295.03
ATTCCTA18650.0295.03
CGATTCC18750.0295.01
TTCCTAA157.1278537E-6294.999974
GATTCCT18800.0294.215422
CCTAGAA18450.0294.200536
CTAGAAC18550.0292.614567
AGAACAG19650.0276.23419
TAGAACA19850.0273.450878
TCCTAAA202.2467191E-5221.255
AGGGGGT452.5465852E-1159.00000420-24
CTGAGGC650.059.00000430-34
GGCACCG900.059.00000435-39
TCTAGGC452.5465852E-1159.00000420-24
GTCTGAG650.059.00000430-34
CTAGGGG2600.059.00000420-24
Produced by FastQC (version 0.11.5)