FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-78-4-2-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-78-4-2-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19193
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC301815.724482884384933No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG19129.961965299848902No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC16918.81050382952118No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG16838.768821966341896No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC10125.272755692179441No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG7874.100453290262075No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC4242.209138748502058No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT4142.157036419527953No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGG3862.0111498984004585No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3541.844422445683322No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3301.7193768561454696No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3281.7089563903506486No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3041.5839108008127964No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG2651.3807117178137862No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGT2471.286927525660397No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1991.0368363465846924No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1500.7815349346115771No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGT1490.7763247017141667No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTG1440.750273537227114No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA1340.6981712082530089No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1190.6200177147918512No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC1070.557494920022925No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1040.5418642213306935No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1040.5418642213306935No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAG1020.5314437555358724No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTG990.515813056843641No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC990.515813056843641No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG900.46892096076694634No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT890.4637107278695357No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTG790.4116083988954306No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT780.4063981659980201No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG770.40118793310060963No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTAGGGGTATCTAATCCCA730.38034700151096756No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA730.38034700151096756No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA690.3595060699213255No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC680.354295837023915No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGCTCGGGGTATCTAATCCCAGTT680.354295837023915No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA680.354295837023915No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC670.34908560412650447No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTG670.34908560412650447No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG660.34387537122909395No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTG640.3334549054342729No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCTCGTGGGGTATCTAATCCCAG520.2709321106653467No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC480.25009117907570466No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA460.23967071328088366No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGT460.23967071328088366No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC460.23967071328088366No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGT450.23446048038347317No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGT430.2240400145886521No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT410.21361954879383108No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGG410.21361954879383108No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC410.21361954879383108No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTTGGGGTATCTAAT410.21361954879383108No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGTGATTTGAGCCGATTGTGGAAC410.21361954879383108No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTTGT350.182358151409368No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC340.1771479185119575No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTG330.17193768561454698No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG320.16672745271713646No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTTG320.16672745271713646No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGC300.15630698692231543No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT280.1458865211274944No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAG260.13546605533267336No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC260.13546605533267336No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC250.13025582243526285No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTTGTG240.12504558953785233No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC230.11983535664044183No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTTG230.11983535664044183No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA210.1094148908456208No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC210.1094148908456208No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA210.1094148908456208No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC200.10420465794821028No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCCGTGGGCTCGGGGTATCTAAT200.10420465794821028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA19050.0294.22574
CTCAATC19050.0294.22572
AATCTAG19100.0293.455475
TCAATCT19100.0293.455473
TCTCAAT19300.0291.943021
TCTAGAA19000.0291.894747
ATCTAGA19400.0288.917546
CTAGAAC19200.0288.08598
TAGAACA20300.0272.475379
CTAGACA600.001781741473.749998
TTAGGGC4750.059.000004115-119
GAGGGAT4500.059.00000425-29
TGAGTTT4500.059.00000450-54
TAGACAG750.004315123459.0000049
GCCCTCG201.8388909E-459.025-29
GTTGATT3950.059.095-99
TTGTTGA4000.059.095-99
TCCTTGG201.8388909E-459.035-39
CAGTTTT3650.059.090-94
GTTGAAG406.0754246E-1059.0105-109
Produced by FastQC (version 0.11.5)