FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-77-5-5-7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-77-5-5-7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18472
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT532228.811173668254657No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT17789.62537895192724No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC16348.845820701602424No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT9605.19705500216544No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA5412.9287570376786487No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT5362.9016890428757036No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3742.024686011260286No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC3101.67821567778259No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2951.5970116933737548No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC2701.46167171935903No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA2531.3696405370290168No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCC2401.29926375054136No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG2331.261368557817237No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2311.2505413598960589No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT2301.2451277609354698No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA1830.990688609787787No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1770.958207016024253No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1200.64963187527068No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1150.6225638804677349No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1080.5846686877436119No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATC980.530532698137722No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCC940.5088783022953659No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCAT880.4763967085318319No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC860.465569510610654No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGC780.42226071892594197No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT740.40060632308358596No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAAT700.37895192724122995No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA690.373538328280641No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCA690.373538328280641No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT670.36271113035946295No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC620.335643135556518No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAG590.319402338674751No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCA570.308575140753573No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT520.281507145950628No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG500.27067994802944995No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCA470.25443915114768295No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT450.24361195322650497No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCAT430.232784755305327No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCA420.22737115634473798No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC420.22737115634473798No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTCAT420.22737115634473798No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC400.21654395842356No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCA390.21113035946297098No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCATC390.21113035946297098No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCA380.205716760502382No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCC380.205716760502382No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT380.205716760502382No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT370.20030316154179298No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCAT370.20030316154179298No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGCTGGGGGTATCTAATCCCAGTCA340.18406236466002598No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATC330.178648765699437No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT320.17323516673884798No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA310.167821567778259No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTC310.167821567778259No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTC300.16240796881767No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCA300.16240796881767No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCATC290.15699436985708098No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTAA280.151580770896492No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT270.14616717193590298No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT260.140753572975314No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGTCGGGGTATCTAATCCCAGTCA260.140753572975314No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCA260.140753572975314No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT260.140753572975314No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCATC250.13533997401472497No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG250.13533997401472497No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCA250.13533997401472497No Hit
GAGACTTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTCAT240.12992637505413598No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCATC230.124512776093547No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTAGTGGGCTCGGGGTATCTAATC220.11909917713295798No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT210.11368557817236899No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTTAAGAGACAGGGGGTATCTAATC200.10827197921178No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT190.102858380251191No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA190.102858380251191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAA18100.0295.05
AGAACGG108.397766E-4295.08
ACTTAGA18150.0295.04
GAGACTT18350.0294.19621
AGACTTA18300.0294.1942
GACTTAG18200.0294.189583
TTAGAAC18150.0293.374666
GAACAGG18100.0293.370189
TAGAACA18750.0283.199987
AGAACAG19050.0278.740178
GACTTAA150.002827111196.666673
TCAGGAA200.006684432147.5295
GCCCTCG303.3087417E-759.00000425-29
CGTTTGT600.059.00000460-64
CGTTCCA150.00436776559.000004215-219
AAACCTC150.00436776559.000004275-279
TTCATTA150.00436776559.000004220-224
GTTTGTG600.059.00000460-64
TGTTGAA600.059.000004105-109
TGGTCCC150.00436776559.000004210-214
Produced by FastQC (version 0.11.5)