FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-76-5-5-7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-76-5-5-7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18311
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT545129.76899131669488No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT18139.901152312817432No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCA13597.421768335972913No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCTG9535.204521872098739No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCA4872.6596035170116323No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCAG4822.6322975260772217No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCCA4592.506689967778931No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT4452.4302331931625796No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT3311.807656599858009No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGCT2411.3161487630386108No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCA2271.23969198842226No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT2121.1577740156190268No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAGC1921.0485500518813826No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1830.999399268199443No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCCA1730.9447872863306209No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1640.8956365026486812No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1570.8574081153405056No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1300.7099557642946863No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCA1110.6061929987439244No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCAG1030.5625034132488668No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT940.513352629566927No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC930.5078914313800448No Hit
AGGAATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCAT830.4532794495112228No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT810.4423570531374583No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTCAT790.4314346567636939No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTCA790.4314346567636939No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC750.40958986401616515No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCAT740.404128665829283No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC730.3986674676424007No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT600.32767189121293216No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC540.2949047020916389No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTCAT530.2894435039047567No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT490.2675987111572279No Hit
AGGAATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTCA470.2566763147834635No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCAG450.2457539184096991No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT430.2348315220359347No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT430.2348315220359347No Hit
AGGAATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATCCAG420.22937032384905248No Hit
AGGAATAGAACAGGCTCCTCTAGACATCGGGGTATCTAATCCCAGTCATC420.22937032384905248No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCATC380.2075255311015237No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAATC370.2020643329146415No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCCA360.19660313472775925No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTCAT350.19114193654087708No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG340.18568073835399487No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT330.18021954016711267No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG320.17475834198023046No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCAT320.17475834198023046No Hit
AGGAATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATCC320.17475834198023046No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAGT320.17475834198023046No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCATCC300.16383594560646608No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCATCC300.16383594560646608No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCCAGC290.15837474741958385No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT280.15291354923270167No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCAT280.15291354923270167No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT270.14745235104581944No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTCA270.14745235104581944No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTAATC270.14745235104581944No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA250.13652995467205506No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT250.13652995467205506No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCATCC240.13106875648517283No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCCA230.12560755829829065No Hit
AGGAATAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTCA230.12560755829829065No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCAT230.12560755829829065No Hit
AGGAATAGAACAGGCTCCTCTAGCGGGGGTATCTAATCCCAGTCATCCAG220.12014636011140843No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGTGTGGGGTATCTAATCCCAGTC210.11468516192452624No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC210.11468516192452624No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT200.10922396373764404No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCATCC200.10922396373764404No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA190.10376276555076185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAGGA108.397208E-4295.02
AAGGACT108.397208E-4295.04
GGAATAG17750.0293.338042
GAACAGG17500.0293.314278
AGGAATA17950.0291.71311
AACAGGC17450.0290.773629
GAATAGA18000.0289.26393
ATAGAAC18050.0284.376745
TAGAACA18350.0278.92376
AATAGAA18750.0276.124
AGAACAG18950.0272.427437
AGGAAGG150.0028269233196.666671
AGGAAAG150.0028269233196.666671
GGAAAAG150.0028269233196.666672
GAAGGAC150.0028269233196.666673
AGACCAG200.0066839894147.57
TATAGAG200.0066839894147.5295
GGAAAGA200.0066839894147.52
AAGCGAA352.0893883E-4126.42858295
AACAGGA352.0893883E-4126.428589
Produced by FastQC (version 0.11.5)