FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-75-4-8-2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-75-4-8-2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18530
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT325817.582298974635727No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG221211.937398812736104No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG13107.069616837560712No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT9935.35887749595251No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG9845.310307609282245No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG7674.1392336751214245No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTG3932.1208850512682136No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3922.1154883971937397No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3401.834862385321101No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT3191.7215326497571504No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGC3181.7161359956826767No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG3021.6297895304910954No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT2461.3275769023205612No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2271.2250404749055586No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1710.9228278467350243No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1480.7987048030221263No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1470.7933081489476526No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGT1460.7879114948731787No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTG1320.712358337830545No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT1320.712358337830545No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT1270.6853750674581759No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTT1210.652995143011333No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1100.5936319481921208No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1090.5882352941176471No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG1050.5666486778197518No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTG990.5342687533729088No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT980.5288720992984349No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT880.4749055585536967No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT820.4425256341068538No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTT770.4155423637344846No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTGT750.404749055585537No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT730.39395574743658934No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA710.38316243928764165No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG660.3561791689152725No Hit
TGCTTATAGAACAGGCTCCTCTAGCACGTCGGGGTATCTAATCCCAGTGT640.3453858607663249No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT610.32919589854290343No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTG600.32379924446842956No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGTTG580.3130059363194819No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCGTGGGGTATCTAATCCCAGTGTG560.30221262817053424No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT540.2914193200215866No Hit
TGCTTATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG540.2914193200215866No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT530.2860226659471128No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT520.28062601187263897No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTTG500.26983270372369134No Hit
TGCTTATAGAACAGGCTCCTCTAGCACGGGGTATCTAATCCCAGTGTGTT500.26983270372369134No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC500.26983270372369134No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTGT500.26983270372369134No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGT490.26443604964921746No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTTG480.2590393955747437No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT470.25364274150026983No Hit
TGCTTATAGAACAGGCTCCTCTAGTTCCGGGGTATCTAATCCCAGTGTGT450.24284943335132217No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGT430.23205612520237454No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGATCGGGGTATCTAATC430.23205612520237454No Hit
TGCTTATAGAACAGGCTCCTCTTAGGGGTATCTAATCCCAGTGTGTTGCT410.2212628170534269No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT400.21586616297895306No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGT390.2104695089044792No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCGGGGTATCTAATCCCAGTG360.19427954668105774No Hit
TGCTTATAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTGTGT360.19427954668105774No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTAGTGGGGTATCTAATCCCAGTG360.19427954668105774No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTGT350.18888289260658392No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTG340.1834862385321101No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATC340.1834862385321101No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTG320.17269293038316244No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAGT310.1672962763086886No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTTG310.1672962763086886No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCCGTGGGGGTATCTAATCCCAGTG300.16189962223421478No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTGTT300.16189962223421478No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGTT300.16189962223421478No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTGT280.15110631408526712No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG280.15110631408526712No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGTCTCGGGGTATCTAATC270.1457096600107933No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT250.13491635186184567No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGGGGGTATCTAATCCCAGTGT240.12951969778737185No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGCGTGTTGCTG230.124123043712898No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT230.124123043712898No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTGT230.124123043712898No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA220.11872638963842418No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT210.11332973556395035No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT210.11332973556395035No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT200.10793308148947653No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT200.10793308148947653No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT200.10793308148947653No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGCTCGGGGTATCTAATCCCAGT200.10793308148947653No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGTG190.10253642741500271No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAA108.397951E-4295.04
TGCTTAT18300.0295.01
AGTCGAA108.397951E-4295.0295
CTTATAG18000.0295.03
GCTTATA18200.0294.189582
TTATAGA18200.0291.758244
GAACAGG18000.0290.90289
TATAGAA18800.0282.446845
ATAGAAC18800.0280.87776
TAGAACA18750.0280.847
AGAACAG19000.0276.368448
CTTATAT150.0028271729196.666673
GCTTATG150.0028271729196.666672
CTTATGA200.006684578147.53
CAACACG600.001780775873.75295
CCAACAG450.00988285965.55556295
TAGCGGG150.004367802359.000004125-129
TCCTTTG5500.059.00000445-49
GTGTTTT150.004367802359.00000450-54
GAGCGGT303.308851E-759.00000485-89
Produced by FastQC (version 0.11.5)