FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-74-4-1-3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-74-4-1-3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18302
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT288315.752376789421922No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTA252513.796306414599496No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCT190510.408698502895858No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT10365.660583542782209No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGA9415.141514588569556No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTA6883.759152005245328No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATC4192.2893672822642332No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT3872.1145230029504973No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2821.5408152114523004No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGAT2781.5189596765380833No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA2631.4370014206097694No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTA2621.431537536881215No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGA2551.3932903507813355No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCG2541.3878264670527811No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2351.2840126762102504No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCG2171.1856627690962735No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTA2031.109168396896514No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1550.8469019779259098No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTAA1500.8195825592831385No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1170.639274396240848No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAA1170.639274396240848No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1160.6338105125122937No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1070.5846355589553054No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTAAC950.5190689542126543No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT950.5190689542126543No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCGA940.5136050704841001No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT870.47535788438422033No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTAA770.4207190470986778No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCG720.3933996284559064No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGA640.3496885586274724No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTA620.3387607911703639No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT600.3278330237132554No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTA600.3278330237132554No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAACC570.31144137252759263No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCG550.30051360507048414No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTAAC540.29504972134192986No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACC510.2786580701562671No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCGA510.2786580701562671No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAA510.2786580701562671No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT510.2786580701562671No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGA500.2731941864277128No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTAACCGA490.26773030269915854No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACCGA490.26773030269915854No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAAC480.2622664189706043No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGT470.25680253524205005No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTA440.24041088405638728No Hit
GAGACTTAGAACAGGCTCCTCTAGCACATGGGGTATCTAATCCCAGTAAC430.234947000327833No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT420.22948311659927878No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGTCAGGGGTATCTAATCCCAGTA400.21855534914217026No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGAT400.21855534914217026No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC390.213091465413616No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTAAC370.20216369795650746No Hit
GAGACTTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTAACCG340.18577204677084472No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA330.18030816304229047No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGACATCGTTTACGGCGTGGACTAC330.18030816304229047No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTAA310.16938039558518195No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACC300.1639165118566277No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT290.15845262812807343No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGCGTGGGTATCTAATCCCAGTAA290.15845262812807343No Hit
GAGACTTAGAACAGGCTCCTCTATACACAGGGTATCTAATCCCAGTAACC280.15298874439951918No Hit
GAGACTTAGAACAGGCTCCTCTAGCTTAGGGGGGTATCTAATCCCAGTAA260.14206097694241065No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGTCGGGGTATCTAATCCCAGT260.14206097694241065No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAACCG250.1365970932138564No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTA240.13113320948530216No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTAACC240.13113320948530216No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT230.12566932575674788No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAACC220.12020544202819364No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGGTATCTAATC220.12020544202819364No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT220.12020544202819364No Hit
GAGACTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAACCG210.11474155829963939No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT210.11474155829963939No Hit
GAGACTTAGAACAGGCTCCTCTAGGTATCGTGGGCTCGGGGTATCTAATC200.10927767457108513No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGTCGGGGTATCTAATCCCAGTAA200.10927767457108513No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGTGTGGGGTATCTAATCCCAGT200.10927767457108513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAA17950.0295.05
GACTTAG18050.0295.03
AGACTTA18150.0293.374662
GAGACTT18150.0293.374661
TTAGAAC18000.0292.541666
ACTTAGA18250.0291.767124
GAACAGG18350.0287.765669
AGAACAG19050.0277.191628
TAGAACA19150.0276.514347
CTTAGAC150.0028269233196.666675
GACGTAG150.0028269233196.666673
GAGACTG150.0028269233196.666671
GACAGGC200.0066839894147.59
CGTAGAA200.0066839894147.55
TTGTTGA3350.059.00000490-94
GGGTCTG650.059.00000425-29
GTTGAGG3300.059.000004105-109
TCTAGGC700.059.00000420-24
AACTGTT3550.059.000004100-104
GGCGCTT650.059.00000465-69
Produced by FastQC (version 0.11.5)