FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-73-4-7-5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-73-4-7-5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17695
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC353219.96044080248658No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG214412.11641706696807No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT12867.267589714608647No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC11326.397287369313364No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCT7284.114156541395875No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGC4402.486578129415089No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTC4192.3679005368748234No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3812.1531506075162476No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3311.8705849109918058No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGC3271.8479796552698502No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3201.8084204577564285No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT2871.621927098050297No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT2861.6162757841198079No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2781.5710652726758971No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCT2181.2319864368465667No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTT1891.0680983328623905No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1520.8589997174343035No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1500.8476970895733259No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1480.8363944617123481No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1420.8024865781294152No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCT1200.6781576716586606No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1200.6781576716586606No Hit
AATCCTATTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGT1090.6159932184232834No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT1080.6103419044927946No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC980.5538287651879062No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTC970.5481774512574173No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC970.5481774512574173No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC890.5029669398135066No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC840.47471037016106243No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA790.44645380050861827No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCG780.44080248657812937No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT720.4068946029951964No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCT670.3786380333427522No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCTCGGGGGTATCTAATCCCAGTC670.3786380333427522No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG660.37298671941226336No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTC630.3560327776207968No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGC610.3447301497598192No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC570.3221248940378638No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA520.2938683243854196No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT500.2825656965244419No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC460.2599604408024866No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA450.2543091268719977No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA440.2486578129415089No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG430.24300649901102003No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGC410.23170387115004237No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCT410.23170387115004237No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCT410.23170387115004237No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGT390.22040124328906469No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG390.22040124328906469No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCT380.21474992935857587No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA380.21474992935857587No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA360.2034473014975982No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG360.2034473014975982No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTCTAGGGAGACAGGGGGGTATCT340.1921446736366205No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATC320.18084204577564283No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC320.18084204577564283No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAA320.18084204577564283No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC310.175190731845154No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT310.175190731845154No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGC300.16953941791466515No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCT290.1638881039841763No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAG290.1638881039841763No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCATGTGTATAAGAGACAGGGGTA280.1582367900536875No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC270.15258547612319864No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCG270.15258547612319864No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTT270.15258547612319864No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGCGGGGTATCTAATCCC260.1469341621927098No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTCG250.14128284826222096No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCG250.14128284826222096No Hit
AATCCTATTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTCGC250.14128284826222096No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGT240.13563153433173214No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT240.13563153433173214No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC230.1299802204012433No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC200.11302627860977678No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC190.10737496467928793No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC190.10737496467928793No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC190.10737496467928793No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGCAGCTCAGGGGTATCTAATC180.1017236507487991No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTA17450.0295.01
CCTATTA17200.0295.04
ATCCATT157.1204377E-6295.02
CCTATAG108.3946734E-4295.04
TCCTATT17250.0295.03
CCATTAG157.1204377E-6295.04
TCCATTA157.1204377E-6295.03
ATCCTAT17500.0293.314272
TATTAGA17350.0292.449556
CTATTAG17350.0292.449555
TTAGAAC17300.0290.7378
ATTAGAA17400.0289.06617
TAGAACA18200.0275.549479
TTAGACA202.2443863E-5221.258
ATTAGAC202.2443863E-5221.257
CATTAGA202.2443863E-5221.255
ACTGCGA150.0028260725196.66667295
TCCTATA255.464795E-5177.03
AATCCAT301.1301474E-4147.51
TAGACAG650.002444335568.076929
Produced by FastQC (version 0.11.5)