FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-72-5-8-2-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-72-5-8-2-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17648
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC593933.65253853127833No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC177710.069129646418858No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG7714.368766999093382No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGC7344.159111514052584No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC5643.1958295557570264No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGT5473.0995013599274706No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4532.5668631006346327No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3912.2155485040797824No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTG2511.4222574796010878No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC2231.263599274705349No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2071.1729374433363553No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1831.036944696282865No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGT1821.0312783318223029No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1791.0142792384406165No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTG1700.9632819582955575No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1680.9519492293744334No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1650.9349501359927471No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1390.7876246600181322No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTG1310.7422937443336356No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG1160.657298277425204No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTG1150.651631912964642No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTT1090.6176337262012692No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1040.5893019038984587No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC890.5043064369900272No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC890.5043064369900272No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT840.4759746146872167No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG830.4703082502266546No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC680.38531278331822305No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGT660.3739800543970988No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC650.36831368993653674No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA620.3513145965548504No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC550.3116500453309157No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC510.2889845874886673No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG480.271985494106981No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTGGGGTATCTAATCCCAGTGTG470.2663191296464189No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC450.25498640072529466No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTG400.22665457842248413No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT400.22665457842248413No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG400.22665457842248413No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC400.22665457842248413No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT390.22098821396192206No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC370.20965548504079784No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC360.20398912058023572No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT340.19265639165911153No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA340.19265639165911153No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG320.1813236627379873No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGT320.1813236627379873No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGT300.1699909338168631No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGT300.1699909338168631No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT270.1529918404351768No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGT270.1529918404351768No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGGGTATCTAA270.1529918404351768No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA270.1529918404351768No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTT260.14732547597461468No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA250.14165911151405258No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCC240.1359927470534905No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT230.13032638259292836No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCA230.13032638259292836No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATTGCGGGGGTATCTAATCCCAGT220.12466001813236627No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC220.12466001813236627No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTG210.11899365367180417No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG210.11899365367180417No Hit
TCTCAATCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG210.11899365367180417No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTGTG210.11899365367180417No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC200.11332728921124206No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACTACCGGGGTATCTAATCCCA190.10766092475067997No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTG190.10766092475067997No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC190.10766092475067997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAG157.120094E-6295.04
CAAGGAG108.394471E-4295.0295
CTCATCT157.120094E-6295.02
TCTCAAT17350.0295.01
CTCAATG108.394471E-4295.02
CTCAATC17250.0294.144932
TCAATCT17200.0294.142463
AATCTAG17200.0293.284885
CAATCTA17400.0289.91384
CTAGAAC17300.0289.88448
TCTAGAA17500.0288.257147
ATCTAGA17550.0287.435886
TAGAACA18050.0277.022169
TCTCATC202.244278E-5221.251
TCTCAAG150.0028260045196.666671
TCATCTA255.4645323E-5177.03
CTCTGGC950.059.00000480-84
TCCTCTA17200.059.00000415-19
CTGAGGC1550.059.00000430-34
TCTAGGG1550.059.00000420-24
Produced by FastQC (version 0.11.5)