FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-71-6-5-8-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-71-6-5-8-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17033
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT881951.77596430458522No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT11706.869018963189104No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT5883.452122350730934No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT4852.8474138437151413No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT4102.4070921153055833No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3622.125286209123466No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCAC2851.6732225679563202No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2631.5440615276228498No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT2561.502964832971291No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT2201.2916104033347033No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1851.0861269300769094No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT1570.9217401514706746No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT1250.7338695473492632No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG950.5577408559854401No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT830.4872893794399108No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT830.4872893794399108No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATCA740.4344507720307638No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT730.4285798156519697No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT700.41096694651558735No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAAT600.35225738272764634No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT450.2641930370457347No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAGGCACTGCCAAGTCCT440.2583220806669406No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT410.24070921153055833No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT400.23483825515176424No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT400.23483825515176424No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT370.2172253860153819No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT370.2172253860153819No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT350.20548347325779368No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT330.19374156050020547No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT330.19374156050020547No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT320.18787060412141138No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT320.18787060412141138No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA310.1819996477426173No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT290.17025773498502908No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAATC260.15264486584864675No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCAA220.1291610403334703No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAAT220.1291610403334703No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA210.12329008395467621No Hit
TGCTTATGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT210.12329008395467621No Hit
TGCTTATAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT210.12329008395467621No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCT210.12329008395467621No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT200.11741912757588212No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTCAA190.111548171197088No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT180.10567721481829391No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCG180.10567721481829391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAC157.1158047E-6295.01
CTTATAG16250.0295.03
GCTTATC157.1158047E-6295.02
TTATGAA108.391948E-4294.999974
TGGAGAA108.391948E-4294.99997295
CTTACTA108.391948E-4294.999973
CTTATGA108.391948E-4294.999973
CTTATCG108.391948E-4294.999973
CATGGAG108.391948E-4294.99997295
GCTTACT108.391948E-4294.999972
TGCTTAT16800.0294.122041
GCTTATA16500.0294.106052
TTATAGA16300.0294.09514
GAACAGG16300.0292.285289
TATAGAA16700.0286.167665
ATAGAAC16800.0282.708346
AGAACAG16950.0280.206488
TAGAACA17100.0277.748547
TTATCGA150.0028251568196.666674
GAAAAGG150.0028251568196.666679
Produced by FastQC (version 0.11.5)