FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-70-5-5-7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-70-5-5-7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16853
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT496329.448762831543345No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT14658.692814335726577No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC14038.324927312644633No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT9695.749718151071026No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA6013.566130659229811No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3922.325995371743903No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3251.9284400403488993No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC2821.6732925888565835No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC2331.3825431673885955No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2191.2994719041120275No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCC2091.2401352874859075No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT2011.1926659941850115No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGC1881.1155283925710555No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA1490.8841155877291876No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1400.8307126327656797No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1390.8247789711030677No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1300.7713760161395598No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1260.7476413694891118No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG1250.7417077078264998No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCATC1140.6764374295377678No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC1140.6764374295377678No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1060.6289681362368718No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCC980.5814988429359758No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT890.5280958879724679No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT810.48062659467157187No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT730.4331573013706759No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCC690.4094226547202279No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT680.4034889930576159No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTC630.3738206847445559No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT620.3678870230819439No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCA610.3619533614193319No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCAT570.3382187147688839No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCA570.3382187147688839No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCA560.3322850531062719No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC550.3263513914436599No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT490.2907494214679879No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCA450.26701477481753993No Hit
TGCTTATAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTCAT430.2551474514923159No Hit
TGCTTATAGAACAGGCTCCTCTAGCACGGGGTATCTAATCCCAGTCATCC420.24921378982970388No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT410.24328012816709194No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT410.24328012816709194No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCA400.23734646650447994No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG400.23734646650447994No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC400.23734646650447994No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG390.2314128048418679No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCAT390.2314128048418679No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGCGGGGTATCTAATCCCAGTC330.1958108348661959No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGCAGGGGTATCTAATCCCAGTCA320.18987717320358394No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT300.17800984987835994No Hit
TGCTTATAGAACAGGCTCCTCTAGTTCTCGTGGGGTATCTAATCCCAGTC300.17800984987835994No Hit
TGCTTATAGAACAGGCTCCTCTAGAACTACCGGGGTATCTAATCCCAGTC300.17800984987835994No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT290.17207618821574794No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTC290.17207618821574794No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT280.16614252655313594No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTAATGTGTATAGTGGGGTATCTAA280.16614252655313594No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT270.16020886489052394No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCCA260.15427520322791194No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA260.15427520322791194No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCCA260.15427520322791194No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTC250.14834154156529994No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA250.14834154156529994No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCAGT250.14834154156529994No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGCAGTCAGGGGTATCTAATCCCA240.14240787990268794No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCA240.14240787990268794No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT240.14240787990268794No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT240.14240787990268794No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAAGTCCT230.13647421824007597No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT230.13647421824007597No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCA220.13054055657746394No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTCAGGGGTATCTAATCCCAGTCA220.13054055657746394No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAGTC210.12460689491485194No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCTCGTGGGCTCGGGGTATCTAATC200.11867323325223997No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCCGTGGGCTCGGAGGGGTATCTAA190.11273957158962795No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT180.10680590992701597No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCA170.10087224826440397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAT16800.0294.122041
TTATAGA16750.0294.119424
CTTATAG16750.0294.119423
GCTTATA16800.0293.244052
GAACAGG16550.0291.435039
TATAGAA17200.0285.566865
AGAACAG17200.0281.279088
ATAGAAC17350.0280.547556
TAGAACA17600.0274.886387
GAAAAGG150.0028249354196.666679
GCCCTCG150.004366450559.00000425-29
CGTTTGT452.5465852E-1159.00000460-64
GAGGCAC2400.059.00000430-34
GTTTGTG452.5465852E-1159.00000460-64
TGTTGAG5250.059.000004105-109
GTTGAGG5250.059.000004105-109
TCCTTAG452.5465852E-1159.00000445-49
GTGTCGC150.004366450559.000004210-214
ATCTTTA150.004366450559.000004220-224
TCTAGTT303.3052675E-759.00000420-24
Produced by FastQC (version 0.11.5)