FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-7-4-5-7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-7-4-5-7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11269
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT201517.880912237110657No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCA131911.704676546277398No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCTG9488.412458958203922No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT5614.978258940456119No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCA5324.7209157866714No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCAG4804.2594728902298336No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCCA4233.753660484515041No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGCT2141.8990149968941346No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCA2071.836897683911616No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCCA1831.6239240394001244No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1781.579554530126897No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAGC1621.437572100452569No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT1301.1536072411039135No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCA1171.038246516993522No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCAG1151.0204987132842311No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT1050.9317596947377762No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCAT910.8075250687727393No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTCAT830.7365338539355755No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTCA830.7365338539355755No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT800.709912148371639No Hit
AGGAATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCAT750.6655426390984116No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC710.6300470316798296No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC670.5945514242612476No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT620.5501819149880203No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT620.5501819149880203No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTCAT520.46144289644156533No Hit
AGGAATAGAACAGGCTCCTCTAGACATCGGGGTATCTAATCCCAGTCATC490.4348211908776289No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCATC480.42594728902298334No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC470.4170733871683379No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCAG460.40819948531369243No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTCAT440.39045168160440147No Hit
AGGAATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATCCAG410.363829976040465No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCAT390.346082172331174No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC390.346082172331174No Hit
AGGAATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTCA380.3372082704765285No Hit
AGGAATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCATCC370.32833436862188303No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCATCC360.31946046676723755No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCCA360.31946046676723755No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT360.31946046676723755No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT320.2839648593486556No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT320.2839648593486556No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAGT310.27509095749401014No Hit
AGGAATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATCC300.2662170556393646No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCAT280.24846925193007366No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG270.23959535007542815No Hit
AGGAATAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTCA270.23959535007542815No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCCAGC250.2218475463661372No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCATCC250.2218475463661372No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC250.2218475463661372No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCCA240.21297364451149167No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTCA240.21297364451149167No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCATCC220.19522584080220073No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCAG200.17747803709290974No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT200.17747803709290974No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC200.17747803709290974No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCATCC200.17747803709290974No Hit
AGGAATAGAACAGGCTCCTCTAGGTTTGGTGGGGTATCTAATCCCAGTCA180.15973023338361877No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT180.15973023338361877No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTAATC180.15973023338361877No Hit
AGGAAGGACTACTGGGGTATCTAATCCCAGTCATCCAGCTGTCTCTTATA170.1508563315289733No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTCA170.1508563315289733No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTATCT170.1508563315289733No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGTGTGGGGTATCTAATCCCAGTC160.1419824296743278No Hit
AGGAATAGAACAGGCTCCTCTATCTGGGGTATCTAATCCCAGTCATCCAG160.1419824296743278No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCAT160.1419824296743278No Hit
AGGAATAGAACAGGCTCCTCTAGCGGGGGTATCTAATCCCAGTCATCCAG160.1419824296743278No Hit
AGGAACATAGTGGGGTATCTAATCCCAGTCATCCAGCTGTCTCTTATACA150.1331085278196823No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTTCAGGGGTATCTAATCCCAGTCA150.1331085278196823No Hit
AGGAATAGAACAGGCTCCTCTAGACAGACTGGGGTATCTAATCCCAGTCA140.12423462596503683No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT130.11536072411039133No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT130.11536072411039133No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGTCGGGGGTATCTAATCCCAGTC130.11536072411039133No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT120.10648682225574584No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAA108.3541445E-4295.02
GGAATAG11150.0293.677122
AGGAATA11300.0292.389371
GAACAGG11000.0289.636358
AACAGGC11000.0289.636359
GAATAGA11400.0285.9433
TAGAACA11500.0279.60876
ATAGAAC11700.0277.350435
AGAACAG11800.0271.257
AATAGAA12050.0270.518654
GCCCTCG150.00435891359.00000420-24
CGTTTGT150.00435891359.00000460-64
GTTTGTG150.00435891359.00000460-64
ACCGGGG303.2853495E-759.00000425-29
GGGTCTG452.5465852E-1159.00000425-29
GGTTGGG452.5465852E-1159.00000420-24
CATGAGC150.00435891359.000004290-294
GGCACCG600.059.00000430-34
TCTAGGG1550.059.00000415-19
GATACTT150.00435891359.00000485-89
Produced by FastQC (version 0.11.5)