FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-69-4-8-2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-69-4-8-2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17408
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC246414.154411764705882No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT216012.408088235294118No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT14578.369715073529411No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT10656.117876838235294No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT8134.670266544117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC7444.27389705882353No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA5333.0618106617647056No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC4272.452895220588235No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3952.2690716911764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTG2701.5510110294117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTT2691.5452665441176472No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT2591.4878216911764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT2551.46484375No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2471.4188878676470589No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC2251.2925091911764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2121.2178308823529411No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG1620.9306066176470588No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTT1490.8559283088235294No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT1450.8329503676470588No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGT1440.8272058823529412No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGT1250.7180606617647058No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG1230.7065716911764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1010.5801930147058824No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1000.5744485294117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTT990.5687040441176471No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTG860.49402573529411764No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTT830.4767922794117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT830.4767922794117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGT800.4595588235294118No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGT730.4193474264705882No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTT730.4193474264705882No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT720.4136029411764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTG720.4136029411764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT720.4136029411764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA690.39636948529411764No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG650.3733915441176471No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCC640.3676470588235294No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT610.35041360294117646No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCAG560.3216911764705882No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG560.3216911764705882No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC550.3159466911764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC540.3102022058823529No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG520.29871323529411764No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTT500.28722426470588236No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG490.2814797794117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA490.2814797794117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTT460.2642463235294118No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT450.2585018382352941No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGT440.25275735294117646No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT430.24701286764705882No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTCGTGGGGTATCTAATCCCAGTGT420.24126838235294118No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC410.2355238970588235No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCA380.2182904411764706No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTG370.21254595588235292No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG370.21254595588235292No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATC370.21254595588235292No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT360.2068014705882353No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC350.20105698529411764No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGT350.20105698529411764No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTCGGGTATCTAATCCCAGT350.20105698529411764No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGTCAGGGGTATCTAATCCCAGTGT320.1838235294117647No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC310.17807904411764705No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTG280.1608455882352941No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGTGGGCTCGGAGGGGTATCTA270.15510110294117646No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTGT250.14361213235294118No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA240.13786764705882354No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG240.13786764705882354No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGT220.12637867647058823No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTG220.12637867647058823No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGC220.12637867647058823No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTGGGGTATCTAATCCCAGTGTGT220.12637867647058823No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC210.12063419117647059No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT200.11488970588235295No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTG190.1091452205882353No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTGT190.1091452205882353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTC17350.0294.149841
GTCTAGA17150.0294.139955
CTAGAAC17200.0293.284887
CGTCTAG17200.0293.284884
CACGTCT17350.0292.449552
TCTAGAA17200.0292.427346
ACGTCTA17400.0291.60923
TAGAACA17900.0279.34368
AGAACAG18050.0276.2059
ACGTCAG150.0028257282196.666673
GATTCAG150.0028257282196.66667295
CGTCTAA150.0028257282196.666674
CGTCAGA200.006681171147.54
AGCCTCC150.004366929659.00000410-14
AGGGGGT501.8189894E-1259.00000420-24
GGGTTTG150.004366929659.00000425-29
GTTGAAG303.3065407E-759.000004105-109
CTGAGGC600.059.00000430-34
TCTAGTT150.004366929659.00000420-24
CCCAGGC150.004366929659.00000475-79
Produced by FastQC (version 0.11.5)