FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-68-4-7-5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-68-4-7-5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16786
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT275416.40652925056595No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCG199211.86703205051829No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC10696.368402239961873No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTCT9765.814369117121411No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT8014.77183367091624No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTTC5323.16930775646372No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCG3652.1744310735136425No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTGT3101.8467770761348743No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCG3031.8050756582866676No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCTG2951.757416895031574No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGCT2841.6918860955558204No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC2421.4416775884665793No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTTC2371.4118908614321457No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT2311.3761467889908259No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCGC2071.233170499225545No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTCT2051.2212558084117717No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT1841.0961515548671512No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC1831.0901942094602646No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTCT1821.084236864053378No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC1560.9293458834743239No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC1440.8578577385916836No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTTC1220.7267961396401764No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG1200.714881448826403No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCGC1150.6850947217919695No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1140.6791373763850828No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCGCTT1000.5957345406886692No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTCT940.559990468247349No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT930.5540331228404624No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTCT890.5302037412129156No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTTC840.5004170141784821No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTCG790.47063028714404864No Hit
ATCTGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG780.46467294173716195No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAGTC730.4348862147027285No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCGC710.4229715238889551No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCTT690.4110568330751817No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATCC690.4110568330751817No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT680.40509948766829496No Hit
ATCTGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCGCTTCTG660.39318479685452157No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCGC660.39318479685452157No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT640.38127010604074824No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTAGGGGTATCTAATCCCAGTC640.38127010604074824No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCGCTT590.35148337900631477No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC560.3336113427856547No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGCTT550.327653997378768No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTCT550.327653997378768No Hit
ATCTGTAGAACAGGCTCCTCTAGGGCTACAGGGGTATCTAATCCCAGTCG550.327653997378768No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTCG550.327653997378768No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCGC510.30382461575122127No Hit
ATCTGTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCTTC500.2978672703443346No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAGTCG470.2799952341236745No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTCG460.2740378887167878No Hit
ATCTGTAGAACAGGCTCCTCTAGCACGGGGGTATCTAATCCCAGTCGCTT450.2680805433099011No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCGC420.25020850708924103No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGCTT400.23829381627546767No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCGCT380.22637912546169428No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTCGC380.22637912546169428No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCGCTT370.2204217800548076No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTCG360.2144644346479209No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGTGGGGGTATCTAATCCCAGTCGC360.2144644346479209No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGTGGGGTATCTAATCCCAGTCGCT360.2144644346479209No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCG330.19659239842726078No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT320.19063505302037412No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGTCGGGGTATCTAATCCCAGTCG310.18467770761348742No Hit
ATCTGTAGAACAGGCTCCTCTAGACGTCAGGGGTATCTAATCCCAGTCGC310.18467770761348742No Hit
ATCTGTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTCGCTTC300.17872036220660076No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTCGC300.17872036220660076No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT290.17276301679971404No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCTTCT280.16680567139282734No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTCGC280.16680567139282734No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCGCT280.16680567139282734No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT270.16084832598594068No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTCGCTTC270.16084832598594068No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCTT270.16084832598594068No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTC270.16084832598594068No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG260.15489098057905398No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC260.15489098057905398No Hit
ATCTGTAGAACAGGCTCCTCTAGGACGGGGTATCTAATCCCAGTCGCTTC260.15489098057905398No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTCTGGGCTCGGAGGGGTATCTAATC250.1489336351721673No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTCGCTT250.1489336351721673No Hit
ATCTGTAGAACAGGCTCCTCTAGACAGGGTATCTAATCCCAGTCGCTTCT230.1370189443583939No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT220.1310615989515072No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTCG200.11914690813773383No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTCT200.11914690813773383No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAGT180.10723221732396045No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT180.10723221732396045No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT180.10723221732396045No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGAGGGTATCTAATC170.10127487191707374No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCGCTT170.10127487191707374No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG108.3908427E-4295.0295
TCTGTAG16800.0293.244022
AACAGGC16600.0293.22299
GAACAGG16700.0291.467048
ATCTGTA16950.0290.648961
CTGTAGA17050.0288.944273
TGTAGAA16950.0288.908544
GTAGAAC16950.0288.038335
AGAACAG17350.0280.547557
TAGAACA17450.0278.939826
CTCTGGG150.0043663659.00000425-29
TGTTGAA303.305031E-759.000004105-109
ACGCCTT150.0043663659.000004180-184
GGTGAAT150.0043663659.00000480-84
GCCTTAG150.0043663659.000004185-189
GGCACCG1550.059.00000430-34
GGCGCCG303.305031E-759.000004210-214
AGCTATT150.0043663659.00000460-64
GAAGTTT303.305031E-759.000004105-109
TCGGTGT150.0043663659.000004200-204
Produced by FastQC (version 0.11.5)