FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-67-5-8-2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-67-5-8-2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16536
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT463928.053942912433477No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT14218.593372036768262No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG13978.448234155781325No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG9065.478955007256895No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG4322.6124818577648767No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT4042.443154329946783No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG4002.418964683115626No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3692.2314949201741654No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2881.741654571843251No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTG2771.6751330430575713No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2521.5239477503628447No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT2521.5239477503628447No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT2111.276003870343493No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1991.2034349298500242No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1711.0341074020319303No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1661.003870343492985No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTT1570.9494436381228833No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGC1550.9373488147073052No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTT1410.8526850507982584No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1350.816400580551524No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1320.7982583454281567No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG1030.6228834059022738No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1020.6168359941944848No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG810.48984034833091433No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG790.4777455249153363No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT760.45960328979196907No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT630.38098693759071117No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA610.368892114175133No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTG550.33260764392839864No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT510.3084179970972424No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT500.3023705853894533No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTTG490.2963231736816643No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTGTTGC450.27213352685050796No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGTG420.2539912917271408No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT410.24794388001935175No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTTG390.2358490566037736No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTTG370.22375423318819546No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTG360.21770682148040638No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGT350.21165940977261732No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGCACATGGGGTATCTAATCCCAGT350.21165940977261732No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT350.21165940977261732No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG330.19956458635703916No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA320.1935171746492501No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC310.18746976294146106No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTCCGGGGTATCTAATCCCAGTGTGT300.18142235123367198No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTG290.1753749395258829No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT270.1632801161103048No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGT270.1632801161103048No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA270.1632801161103048No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGTTG270.1632801161103048No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT250.15118529269472664No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT250.15118529269472664No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT240.14513788098693758No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTGCAGGGGTATCTAATCCCAGTG240.14513788098693758No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAGTG230.13909046927914853No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTG220.13304305757135945No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT220.13304305757135945No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGT220.13304305757135945No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT210.1269956458635704No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTGT210.1269956458635704No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGT210.1269956458635704No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT200.12094823415578133No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCC200.12094823415578133No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT190.11490082244799227No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGTG190.11490082244799227No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT190.11490082244799227No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT180.10885341074020319No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGTT180.10885341074020319No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTATC180.10885341074020319No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCCGGGGGTATCTAATCCCAGTG180.10885341074020319No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT180.10885341074020319No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT180.10885341074020319No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAATCC170.10280599903241412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATA108.389703E-4295.04
GGTAGAA16050.0294.0815
TGGTAGA16250.0293.18464
GTGGTAG16350.0292.293583
GAACAGG16200.0291.358039
GAGTGGT16700.0288.817381
AGTGGTA16650.0288.798772
GTAGAAC16350.0288.6856
TAGAACA17050.0276.832867
AGAACAG17100.0276.02348
GTGGTAT150.0028244038196.666663
CCGCCAG5000.059.00000435-39
AGTGTTC5700.059.00000470-74
GGCTCCT16250.059.00000410-14
TGTTGAT5500.059.090-94
TGTTGAA257.77023E-659.0105-109
GGGTTTT1750.059.050-54
GGGTTTG501.8189894E-1259.025-29
GGGTTTA201.8373899E-459.0110-114
GAGCGGT501.8189894E-1259.085-89
Produced by FastQC (version 0.11.5)