FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-66-4-2-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-66-4-2-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16501
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG245414.871825949942426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC166210.07211684140355No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC10906.605660263014363No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG8425.1027210472092595No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG8184.957275316647476No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT5703.4543361008423727No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC4232.5634810011514455No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3912.3695533604023997No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC3762.278649778801285No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG2761.6726259014605176No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGG2611.5817223198594026No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGT2411.460517544391249No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT2321.4059753954305798No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTT2201.3332525301496878No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1901.1514453669474578No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1811.0969032179867886No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1751.0605417853463426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC1380.8363129507302588No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC1370.8302527119568512No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGT1300.7878310405429974No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA1270.7696503242227744No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1210.7332888915823284No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT1160.7029876977152899No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGGGCTCGGAGGGGTATCT1140.6908672201684747No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1130.684806981395067No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG1090.6605660263014363No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1030.6242045936609902No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTG1010.6120841161141749No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC990.5999636385673596No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT960.5817829222471366No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA930.5636022059269135No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG890.5393612508332828No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTG820.4969395794194292No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT800.4848191018726138No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC790.4787588630992061No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACATCGGGGTATCTAATCCCAGTT790.4787588630992061No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTT760.4605781467789831No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT760.4605781467789831No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGCTCGGGGGTATCTAATCCCAGT730.44239743045876007No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTG730.44239743045876007No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG670.40603599781831407No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT650.3939155202714987No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAG610.369674565177868No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA550.33331313253742195No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC540.3272528937640143No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTG520.31513241621719895No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTTG500.3030119386703836No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGT490.29695169989697595No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTTG470.2848312223501606No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA460.2787709835767529No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC460.2787709835767529No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTG460.2787709835767529No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTT450.2727107448033453No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTG440.26665050602993756No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC430.26059026725652995No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAAT400.2424095509363069No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTGCAGCACGCGGAGGGGGTAT400.2424095509363069No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTTG390.23634931216289923No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG390.23634931216289923No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT350.2121083570692685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG350.2121083570692685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTTGT330.19998787952245317No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTG320.19392764074904553No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC320.19392764074904553No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTTGT300.18180716320223017No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTAAAGTCATGTATCTACGGTAGG290.17574692442882248No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTTGTGG280.1696866856554148No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAG280.1696866856554148No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCA280.1696866856554148No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGG280.1696866856554148No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTG270.16362644688200714No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCC270.16362644688200714No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACCGGGGTATCTAATCCCAGTTG260.15756620810859948No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGT260.15756620810859948No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC260.15756620810859948No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT250.1515059693351918No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGT240.14544573056178414No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA240.14544573056178414No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACCAAGGGGTATCTAATCCCAGTT230.13938549178837645No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTA220.13332525301496878No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC220.13332525301496878No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTG210.12726501424156111No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGGTATCTAATCC200.12120477546815345No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA200.12120477546815345No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCA200.12120477546815345No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATC190.11514453669474578No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG180.1090842979213381No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCA180.1090842979213381No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTG170.10302405914793043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAGC16200.0295.02
CTCGCTA108.389703E-4295.03
TTCTCAG16250.0294.092321
CTCAGCT16150.0294.086673
TCAGCTA16200.0293.179024
CAGCTAG16200.0292.268525
GCTAGAA16300.0291.380377
AGCTAGA16300.0290.475466
CTAGAAC16650.0285.255258
TAGAACA17400.0272.959789
TTCTCAA150.0028244038196.666661
AGCTAAG200.006678046147.5295
CGCTAGA200.006678046147.55
TCGCTAG200.006678046147.54
TTCTCGC200.006678046147.51
CTCAGCA200.006678046147.53
CGATGAG200.006678046147.5295
TAGGCAG200.006678046147.5295
TCCTCTA16200.059.00000415-19
CTCTAGG11450.059.00000420-24
Produced by FastQC (version 0.11.5)