FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-65-5-5-7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-65-5-5-7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15907
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC470529.57817313132583No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG177211.139749795687433No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC14288.977179857924185No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG7634.79663041428302No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATC5243.2941472307789024No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3662.3008738291318287No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3382.124850694662727No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCC2671.6785063179732194No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT2631.6533601559062048No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCAT2471.5527755076381469No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG2101.3201735085182624No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2061.2950273464512478No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1871.175583076632929No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATC1560.9807003206135664No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1400.8801156723455084No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1370.8612560507952474No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA1280.8046771861444648No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1260.7921041051109575No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1030.6475136732256239No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT950.5972213490915949No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA830.5217828628905513No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCAT800.5029232413402904No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC780.4903501603067832No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA700.4400578361727542No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC650.408625133588986No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT630.39605205255547876No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC620.38976551203872506No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATC550.3457597284214497No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC550.3457597284214497No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT530.3331866473879424No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCC520.3269001068711888No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATC510.32061356635443516No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT460.289180863770667No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC460.289180863770667No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATC380.23888853963663798No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA380.23888853963663798No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC360.2263154586031307No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT360.2263154586031307No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC350.2200289180863771No Hit
AATCCTATTAGAACAGGCTCCTCTAGCACGGGGGTATCTAATCCCAGTCA350.2200289180863771No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATC350.2200289180863771No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG350.2200289180863771No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCT350.2200289180863771No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTC320.2011692965361162No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT310.19488275601936253No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCA310.19488275601936253No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTC310.19488275601936253No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC290.1823096749858553No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT270.16973659395234802No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAG260.1634500534355944No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA260.1634500534355944No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTAGATCTCCTTGGGGTATCT250.15716351291884076No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC250.15716351291884076No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC240.15087697240208714No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTATCGTGGGCTCGGGGTATCTAA230.1445904318853335No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATC230.1445904318853335No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCA220.13830389136857987No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCA220.13830389136857987No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC220.13830389136857987No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG220.13830389136857987No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT210.13201735085182625No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT200.1257308103350726No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG190.11944426981831899No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCGGGGTATCTAATCCCAG180.11315772930156535No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC170.10687118878481172No Hit
AATCCTATTAGAACAGGCTCCTCTAGACCCTCGATCTCCTTGGGGTATCT160.1005846482680581No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC160.1005846482680581No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAG160.1005846482680581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTA15250.0295.000034
CCTATTG108.3868264E-4295.04
CCTATAG108.3868264E-4295.04
ATTAGAA15150.0295.07
TATTGAA108.3868264E-4295.06
ATCCTAT15550.0294.051452
TCCTATT15500.0293.096743
TTAGAAC15400.0292.126658
AATCCTA15750.0291.253971
TATTAGA15300.0291.143776
CTATTAG15300.0291.143775
TAGAACA16500.0273.545479
CAGAACC150.002823437196.66667295
CTATTGA150.002823437196.666675
CTATTAA255.454602E-5177.05
TCCTATA200.006675768147.53
TTGAACA200.006675768147.58
CAGAGAA703.0630195E-584.28571295
TATTAAA600.001776129273.756
GCCCTCG150.004365545759.00000425-29
Produced by FastQC (version 0.11.5)