FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-64-4-1-3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-64-4-1-3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16202
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT299618.491544253795826No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTAA215613.306999135909148No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCTG13868.554499444513024No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT10826.678187878039749No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGAT6363.9254413035427724No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTAA3452.1293667448463154No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCGA3121.9256881866436242No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATCT2991.8454511788668064No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT2871.7713862486112824No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGAT2751.6973213183557587No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTAA2611.6109122330576473No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2391.4751265275891865No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCGA1981.2220713492161461No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTA1821.1233181088754474No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1741.0739414887050982No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGATC1671.0307369460560425No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTAAC1500.92581162819405No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1340.8270583878533515No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCGA1130.6974447599061845No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG1070.6604122947784224No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTAA1010.6233798296506604No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAAC1000.6172077521293667No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT880.5431428218738427No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACCGAT830.5122824342673744No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT830.5122824342673744No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTAACC790.4875941241821997No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCGAT700.43204542649055677No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTA690.4258733489692631No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAAC580.35798049623503275No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTAA570.3518084187137391No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT550.33946426367115173No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTAAC550.33946426367115173No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTAA540.33329218614985806No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTAAC530.3271201086285644No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTAAC520.3209480311072707No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC510.31477595358597704No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAACC480.296259721022096No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA480.296259721022096No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA450.277743488458215No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT440.27157141093692133No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA430.2653993334156277No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT430.2653993334156277No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACCG430.2653993334156277No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTA390.24071102333045302No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCGA370.22836686828786568No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT360.222194790766572No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG360.222194790766572No Hit
ATCTGTAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTAACCG360.222194790766572No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTATTGGGGTATCTAATCCCAGTAA350.21602271324527839No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTAA350.21602271324527839No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTAAC340.2098506357239847No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAAC320.19750648068139737No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT320.19750648068139737No Hit
ATCTGTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTAACC320.19750648068139737No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTAACC320.19750648068139737No Hit
ATCTGTAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATCCC300.18516232563881002No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACCG300.18516232563881002No Hit
ATCTGTAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTAACCG300.18516232563881002No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCCC300.18516232563881002No Hit
ATCTGTAGAACAGGCTCCTCTAGCGAGTCGTATGGGGTATCTAATCCCAG290.17899024811751638No Hit
ATCTGTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTAACCGAT280.1728181705962227No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTA270.16664609307492903No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCAG270.16664609307492903No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCACGGAGGGGTATCTAA270.16664609307492903No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCTCGTGGGCTCGGGGTATCTAATCC260.16047401555363536No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAACCG250.15430193803234168No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGAT240.148129860511048No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT230.14195778298975434No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTAACC230.14195778298975434No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTAACC230.14195778298975434No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT210.12961362794716702No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG200.12344155042587335No Hit
ATCTGTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTAAC200.12344155042587335No Hit
ATCTGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG200.12344155042587335No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGAT200.12344155042587335No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACCG200.12344155042587335No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT190.11726947290457967No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTAAC180.111097395383286No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGTGGGCTCGGGGGTATCTAATC180.111097395383286No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT180.111097395383286No Hit
ATCTGTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTAA180.111097395383286No Hit
ATCTGTAGAACAGGCTCCTCTAGATTCGGGGTATCTAATCCCAGTAACCG180.111097395383286No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTA170.10492531786199236No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA170.10492531786199236No Hit
ATCTGTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTAA170.10492531786199236No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAACCG170.10492531786199236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTAG15950.0294.075232
ATCTGTA16100.0292.251561
CTGTAGA16050.0292.242983
AACAGGC15900.0292.216989
GAACAGG16100.0290.419258
TAGAACA16750.0279.149266
AGAACAG16900.0278.417187
GTAGAAC17050.0275.102635
TGTAGAA17050.0275.102634
TCTGAGA150.0028239295196.666662
CAATAGG200.006676928147.5295
GGGTTTT1250.059.00000450-54
TCCTTAG700.059.00000445-49
GGCATAG1800.059.000004120-124
TCTAGGC452.5465852E-1159.00000415-19
GGACTAC1400.059.00000420-24
GCGGTTT351.4118996E-859.00000485-89
CCGCCAG3350.059.00000435-39
GTATTTT452.5465852E-1159.00000495-99
GGTTTTA1250.059.00000450-54
Produced by FastQC (version 0.11.5)