FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-63-5-8-2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-63-5-8-2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15889
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT505931.839637485052553No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT169010.636289256718484No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG10886.847504562905154No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG6664.191579079866575No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG4793.014664233117251No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT4542.8573226760652024No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT4232.662219145320662No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG4152.6118698470640065No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT2571.6174712064950594No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTG1941.220970482723897No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1921.208383158159733No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1480.9314620177481278No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT1420.893700044055636No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG1360.8559380703631443No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGC1320.8307634212348165No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1280.8055887721064888No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT1220.767826798413997No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT1000.6293662282081943No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT900.566429605387375No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT770.48461199572030966No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT760.4783183334382277No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT650.40908804833532636No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA640.4027943860532444No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTT630.3965007237711624No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT630.3965007237711624No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT620.3902070614890805No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT620.3902070614890805No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTG590.3713260746428347No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA560.35244508779658884No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGT540.33985776323242495No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG530.333564100950343No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTT510.3209767763861791No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT410.2580401535653597No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT410.2580401535653597No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTG410.2580401535653597No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG390.2454528290011958No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTG390.2454528290011958No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT370.23286550443703194No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA360.22657184215494997No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT340.21398451759078607No Hit
TGCTTATAGAACAGGCTCCTCTAGCACGGGGTATCTAATCCCAGTGTGTT340.21398451759078607No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT330.20769085530870413No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT310.19510353074454026No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCGTGGGGTATCTAATCCCAGTGTG290.18251620618037637No Hit
TGCTTATAGAACAGGCTCCTCTAGTTCCGGGGTATCTAATCCCAGTGTGT290.18251620618037637No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTTG280.17622254389829442No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT280.17622254389829442No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTGT280.17622254389829442No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT270.16992888161621247No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTGT260.16363521933413055No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT260.16363521933413055No Hit
TGCTTATAGAACAGGCTCCTCTAGCACGTCGGGGTATCTAATCCCAGTGT250.15734155705204858No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTTG250.15734155705204858No Hit
TGCTTATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG250.15734155705204858No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT250.15734155705204858No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA240.15104789476996663No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTG230.1447542324878847No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGTTG220.13846057020580274No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT220.13846057020580274No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCCCGTGGGCTCGGGGTATCTAATC220.13846057020580274No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGT220.13846057020580274No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATC210.13216690792372082No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT210.13216690792372082No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT200.12587324564163888No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGT200.12587324564163888No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT200.12587324564163888No Hit
TGCTTATAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTGTGT190.11957958335955693No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGT190.11957958335955693No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT180.11328592107747498No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG180.11328592107747498No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTGT170.10699225879539304No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTTG170.10699225879539304No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGATCGGGGTATCTAATC170.10699225879539304No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAGGTCCT160.1006985965133111No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT160.1006985965133111No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGTT160.1006985965133111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAT15800.0295.01
CTTATGA108.3865755E-4295.03
CTTATAG15650.0295.03
GCTTATG108.3865755E-4295.02
TTATAGA15650.0294.057534
GCTTATA15750.0293.126982
GAACAGG15400.0293.084449
TATAGAA16000.0286.703125
TAGAACA16150.0284.040257
AGAACAG16100.0283.090068
ATAGAAC16250.0282.29236
ATAGAGG150.002823353196.66667295
GGGTCTG2150.059.00000425-29
GAGCGGT452.5465852E-1159.00000485-89
TCTAGTC303.302739E-759.00000420-24
ATCTTGG150.004365494559.000004200-204
GTCACAG150.004365494559.000004290-294
GGGCACC303.302739E-759.00000430-34
TCTAGGC150.004365494559.00000420-24
CTTTAGG150.004365494559.000004110-114
Produced by FastQC (version 0.11.5)