FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-62-4-5-7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-62-4-5-7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15927
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT266716.745149745714823No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC171710.780435738054875No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC10026.291203616500282No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCC8305.21127644879764No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATC6213.899039367112451No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA5453.4218622464996544No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT5443.4155836001758018No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG4362.7374897971997236No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCA3962.486343944245621No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAG3752.3544923714447163No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3212.0154454699566773No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC2681.6826772147924909No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC2581.6198907515539651No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATC2431.5257110566961762No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT2431.5257110566961762No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCAT2281.4315313618383876No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1981.2431719721228105No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCA1611.010862058140265No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCAT1570.9857474728448546No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCA1260.7911094368054248No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTC1180.7408802662146041No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT1130.7094870345953412No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATC960.6027500470898475No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCC950.5964714007659948No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC940.5901927544421423No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC910.5713568154705846No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT820.5148489985559114No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC820.5148489985559114No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCC820.5148489985559114No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGT770.4834557669366485No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCC750.4708984742889433No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCC740.4646198279650907No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCAT730.4583411816412381No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC710.445783888993533No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCC690.43322659634582783No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTCA680.4269479500219752No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA630.3955547184027124No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT620.3892760720788598No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAGT590.37044013310730206No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT580.3641614867834495No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC560.35160419413574434No Hit
CGATTCCTAGAACAGGCTCCTCTAAGGACTACCGGGGTATCTAATCCCAG520.32648960884033407No Hit
CGATTCCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA520.32648960884033407No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG510.32021096251648146No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGT510.32021096251648146No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTCATC490.3076536698687763No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTCGCGTCAGGGGTATCTAATCC480.30137502354492374No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCA460.28881773089721857No Hit
CGATTCCTAGAACAGGCTCCTCTAAGTTGGGGTATCTAATCCCAGTCATC440.2762604382495134No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC440.2762604382495134No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC390.2448672066302505No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCATC380.23858856030639794No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC330.20719532868713506No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC320.20091668236328247No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCAT320.20091668236328247No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGCGGGGTATCTAATCCCAGTCA310.1946380360394299No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCAT310.1946380360394299No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGATTCTTGGGGTATCTAATCCCA280.17580209706787217No Hit
CGATTCCTAGAACAGGCTCCTCTTATACGGGGTATCTAATCCCAGTCATC280.17580209706787217No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCAT270.1695234507440196No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAGT270.1695234507440196No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCCTGGGGTATCTAATCCCAGT260.16324480442016703No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG260.16324480442016703No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTC260.16324480442016703No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATC250.15696615809631442No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCAT250.15696615809631442No Hit
CGATTCCTAGAACAGGCTCCTCTTAGGGTATCTAATCCCAGTCATCCAGC230.14440886544860929No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC230.14440886544860929No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGT220.1381302191247567No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT190.11929428015319897No Hit
CGATTCCTAGAACAGGCTCCTCTAGACCATTTCGGGGGGTATCTAATCCC170.1067369875054938No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC170.1067369875054938No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGTC160.10045834118164124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG108.3868264E-4295.04
TTCCTAG15700.0295.04
GATTCGT108.3868264E-4295.02
GATTCCT15750.0295.02
CGATTCC15800.0294.066471
TCCTAGA15700.0294.060525
CCTAGAA15650.0294.057536
ATTCCTA15800.0293.13293
CTAGAAC15800.0288.465187
AGAACAG16250.0279.56929
TAGAACA16450.0277.066868
AAGTGGG150.002823437196.66667295
CGATTCG150.002823437196.666671
ATTCGTA200.006675768147.53
TCGTAGA200.006675768147.55
TAGAAAA508.611382E-488.58
TGTTGAA150.004365545759.000004105-109
GAGCGGT150.004365545759.00000485-89
TCCTTAG150.004365545759.00000445-49
GGTTGGG150.004365545759.00000425-29
Produced by FastQC (version 0.11.5)