FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-61-5-2-6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-61-5-2-6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15685
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC520833.203697800446285No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC167710.691743704175964No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG8915.680586547656997No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC6153.920943576665604No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC5873.742429072362129No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4352.7733503347146957No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG4322.7542237806821803No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG2721.7341408989480396No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC2641.6831367548613325No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2031.2942301562001912No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT1951.2432260121134842No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG1931.2304749760918074No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1560.9945808096907874No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC1380.8798214854956965No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1300.8288173414089895No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGT1130.7204335352247371No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1120.7140580172138986No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1120.7140580172138986No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGT1030.6566783551163532No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC950.6056742110296461No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGG860.5482945489321007No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA840.535543512910424No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT780.4972904048453937No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT770.4909148868345553No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA770.4909148868345553No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC770.4909148868345553No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC640.40803315269365636No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTG580.36978004462862607No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT510.3251514185527574No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG500.318775900541919No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGT490.31240038253108066No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT470.2996493465094039No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA440.2805227924768887No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTT430.27414727446605036No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC410.2613962384443736No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC410.2613962384443736No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTG400.2550207204335352No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC380.24226968441185848No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC370.23589416640102007No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC360.22951864839018168No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTG330.21039209435766656No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCTCGGGGTATCTAATCCCAGT320.20401657634682818No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC310.19764105833598983No Hit
AATCCTATTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT310.19764105833598983No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTG280.17851450430347465No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT280.17851450430347465No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC260.1657634682817979No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC250.1593879502709595No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTT240.15301243226012112No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTAGGGGTATCTAA240.15301243226012112No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG240.15301243226012112No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG230.14663691424928277No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT230.14663691424928277No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTG230.14663691424928277No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC220.14026139623844436No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTTGT220.14026139623844436No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG220.14026139623844436No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC210.133885878227606No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTGCTCGGGGTATCTAATCCCAG210.133885878227606No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTG200.1275103602167676No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA200.1275103602167676No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC200.1275103602167676No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT200.1275103602167676No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTT200.1275103602167676No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGGGGGTATCTAATCCCAGTTGTG190.12113484220592924No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATC190.12113484220592924No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCGGGGTATCTAATCCCAGTTGT190.12113484220592924No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTT180.11475932419509084No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT180.11475932419509084No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC170.10838380618425247No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT170.10838380618425247No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGTGATTTGAGCCGATTGTGGAAC160.10200828817341409No Hit
AATCCTATTAGAACAGGCTCCTCTAGATCTCGGGGGTATCTAATCCCAGT160.10200828817341409No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTTG160.10200828817341409No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG160.10200828817341409No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTG160.10200828817341409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT15600.0295.02
CCTATAG108.3855615E-4295.04
TCCTATT15450.0295.03
TCCTATA157.1049617E-6295.03
CCTATTA15400.0294.999974
AATCCTA15800.0292.199371
ATTAGAA15450.0292.135937
TATTAGA15550.0291.20586
CTATTAG15550.0291.20585
TTAGAAC15500.0290.241948
TAGAACA16000.0279.328129
TGAGCAA200.0066747665147.5295
CAACGAA858.0153135E-569.411766295
GGGTCTG1750.059.00000425-29
CTGAGGC1750.059.00000430-34
TCTAGTC452.5465852E-1159.00000420-24
GATACTT452.5465852E-1159.00000490-94
TTGTAGT2750.059.00000470-74
GGTCTGA1750.059.00000430-34
GGTTTGG452.5465852E-1159.00000430-34
Produced by FastQC (version 0.11.5)