FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-60-5-2-6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-60-5-2-6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15432
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC445428.862104717470196No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC11337.341886988076723No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT9536.175479523068947No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT7985.171073094867807No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG5283.421461897356143No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4452.8836184551581128No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC3892.5207361327112494No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT3202.0736132711249353No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTG2811.8208916537065838No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2111.3672887506480043No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGG2031.3154484188698807No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCC1911.2376879212026957No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1761.1404872991187145No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1731.121047174701918No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1561.010886469673406No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1420.92016588906169No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTT1390.9007257646448937No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT1210.784085018144116No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT1180.7646448937273198No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1100.7128045619491965No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGC1070.6933644375324002No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTG1030.6674442716433385No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTT1010.6544841886988076No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA990.6415241057542769No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG960.6220839813374806No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG930.6026438569206842No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC840.5443234836702955No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG760.49248315189217207No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTGT680.44064282011404876No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTT660.4276827371695179No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG650.42120269569725244No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC630.4082426127527216No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT600.38880248833592534No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT570.3693623639191291No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC550.35640228097459825No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTT490.31752203214100566No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA470.3045619491964749No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATC470.3045619491964749No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTTG470.3045619491964749No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA450.291601866251944No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC450.291601866251944No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTTG380.24624157594608603No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGG370.23976153447382065No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTG360.23328149300155523No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTT360.23328149300155523No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTCTCTGTGGGGGTATCTAATCCCA350.2268014515292898No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC340.22032141005702438No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTAGGGGTATCTAATCCCAG330.21384136858475894No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC330.21384136858475894No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT330.21384136858475894No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG300.19440124416796267No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTTGT290.18792120269569726No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGTCACCAGGGGTATCTAATCCCA280.18144116122343182No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC280.18144116122343182No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA270.1749611197511664No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTT260.168481078278901No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGG260.168481078278901No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGTCGGGGGTATCTAATCCCAGTT250.16200103680663558No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTT240.15552099533437014No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT240.15552099533437014No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT240.15552099533437014No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTGT240.15552099533437014No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC240.15552099533437014No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTT220.1425609123898393No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTCAGGGGTATCTAATCCCAGT220.1425609123898393No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAGT210.13608087091757387No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTTGT200.12960082944530846No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTTGT190.12312078797304302No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC190.12312078797304302No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTGT190.12312078797304302No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGG180.11664074650077762No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC180.11664074650077762No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGG170.11016070502851219No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC170.11016070502851219No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT170.11016070502851219No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC160.10368066355624676No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC160.10368066355624676No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACGG108.3842577E-4294.999979
CCACGTC15350.0294.039061
CACGTCT15300.0294.035922
TCTAGAA15200.0294.02966
CGTCTAG15250.0293.065554
CTAGAAC15200.0293.05927
ACGTCTA15300.0292.107823
GTCTAGA15400.0290.211065
AGAACAG15500.0284.532239
TAGAACA15650.0281.80518
GACATGA200.006673734147.49998295
AGCAGTT5000.059.00000485-89
CCTCTAG15150.059.00000415-19
AGCACCG5750.059.00000435-39
CGTTTCA201.836616E-459.0215-219
AGGGGGT201.836616E-459.020-24
TTTTGAG201.836616E-459.050-54
GGGTTTT1500.059.050-54
GAGCGGT501.8189894E-1259.085-89
TCCTTAG351.4106263E-859.045-49
Produced by FastQC (version 0.11.5)