FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-6-4-4-1-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-6-4-4-1-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11230
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT203018.07658058771149No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT131111.674087266251112No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG10709.528049866429207No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT5144.5770258236865535No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC4894.354407836153161No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG3453.0721282279608193No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCC3072.7337488869100626No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCT2482.2083704363312555No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC2382.119323241317898No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG2011.7898486197684773No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACC1991.772039180765806No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG1961.7453250222617986No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT1881.674087266251113No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT1401.2466607301869992No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1231.0952804986642921No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA900.8014247551202136No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT830.7390917186108638No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT790.703472840605521No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT710.6322350845948354No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG690.6144256455921638No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT560.4986642920747996No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACC540.4808548530721282No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT530.4719501335707925No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT530.4719501335707925No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCT520.4630454140694568No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT510.45414069456812106No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT510.45414069456812106No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTG500.44523597506678536No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT500.44523597506678536No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTAC460.40961709706144256No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTACC430.38290293855743546No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCC420.37399821905609976No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCC420.37399821905609976No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT400.3561887800534283No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTAC390.3472840605520926No Hit
TGCTTATAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTG390.3472840605520926No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTG390.3472840605520926No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGTATCTAATCCCAGTGTACC380.3383793410507569No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACC370.3294746215494212No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTGTA350.3116651825467498No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTA350.3116651825467498No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT330.2938557435440784No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT320.28495102404274264No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT320.28495102404274264No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC290.25823686553873554No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCC270.2404274265360641No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT270.2404274265360641No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTGTAC270.2404274265360641No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTAC260.2315227070347284No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA260.2315227070347284No Hit
TGCTTATAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTGT250.22261798753339268No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTG250.22261798753339268No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGTA240.21371326803205698No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT230.20480854853072128No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGTA230.20480854853072128No Hit
TGCTTATAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTGTAC220.19590382902938555No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT220.19590382902938555No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTG220.19590382902938555No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT220.19590382902938555No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTAC210.18699910952804988No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCAGT210.18699910952804988No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTGTAC210.18699910952804988No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGCGGCACGCAGGGGTATCTAATCC210.18699910952804988No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA200.17809439002671415No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTAC190.16918967052537845No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACCC190.16918967052537845No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGTGT190.16918967052537845No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACCC180.16028495102404272No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT180.16028495102404272No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGT180.16028495102404272No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAGCAGCGGGGTATCTAATCCC180.16028495102404272No Hit
TGCTTATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCT180.16028495102404272No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTACC170.15138023152270705No Hit
TGCTTATAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTGTAC170.15138023152270705No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTACC160.14247551202137132No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTGT160.14247551202137132No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCC150.13357079252003562No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG150.13357079252003562No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT140.1246660730186999No Hit
TGCTTATAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTGTACCC140.1246660730186999No Hit
TGCTTATAGAACAGGCTCCTCTAGAACGAGAATGGGGTATCTAATCCCAG130.1157613535173642No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT130.1157613535173642No Hit
TGCTTATAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTACC120.10685663401602849No Hit
TGCTTATAGAACAGGCTCCTCTAGCGCTCTTTCGATCTTGGGGTATCTAA120.10685663401602849No Hit
TGCTTATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG120.10685663401602849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGA11000.0295.04
GCTTATG108.353645E-4295.02
TGCTTAT11200.0293.683041
CTTATAG11050.0293.665163
GCTTATA11150.0292.354252
TATAGAA11300.0287.168125
GAACAGG11300.0285.862829
TAGAACA11400.0284.649147
ATAGAAC11500.0282.173926
AGAACAG11800.0275.08
GAGCAGG150.0028122955196.66667295
CTTATGA150.0028122955196.666673
TTATGAA200.00664949147.54
TATGAAC200.00664949147.55
TTTGAGT2750.059.00000450-54
TTTCTAT452.5465852E-1159.000004100-104
GTTCTGG2750.059.00000475-79
TGTTCTG2750.059.00000475-79
AGGGCTA2750.059.000004115-119
TTGAGTT2750.059.00000450-54
Produced by FastQC (version 0.11.5)