FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-59-4-5-7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-59-4-5-7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15279
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC251216.44086654885791No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT210713.79016951371163No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC157710.321356109693044No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC7164.686170560900583No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCC6394.1822108776752405No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT5603.6651613325479415No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATC3812.4936186923227956No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3802.4870737613718172No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAG2161.413705085411349No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC2121.387525361607435No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATC2011.3155311211466718No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCC1891.2369919497349302No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1791.1715426402251456No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCA1771.1584527783231886No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC1671.093003468813404No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCA1611.0537338831075331No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1460.9555599188428563No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1430.9359251259899208No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1390.9097454021860069No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1250.8181163688723083No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG1130.7395771974605667No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC970.6348583022449114No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCAT890.5824988546370835No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG890.5824988546370835No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC890.5824988546370835No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCAT860.5628640617841482No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCA790.517049545127299No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC710.4646900975194712No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC690.4516002356175142No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA650.4254205118136004No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC640.4188755808626219No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT630.4123306499116434No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCAT600.3926958570587081No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC580.3796059951567511No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC570.3730610642057726No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT560.36651613325479415No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCA560.36651613325479415No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT550.3599712023038157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATCC550.3599712023038157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA550.3599712023038157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCAT510.3337914784999019No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCC470.30761175469598795No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG470.30761175469598795No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCC450.294521892794031No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATC430.2814320308920741No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC410.26834216899011715No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC400.2617972380391387No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGT380.24870737613718175No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCC360.23561751423522484No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGTGCGGGGGTATCTAATCCCAGTC350.22907258328424634No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC340.22252765233326788No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGCTACTCGGGGTATCTAATCCCAG340.22252765233326788No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG310.20289285948033248No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGAAATAACAGGGGTATCTAATCCCA310.20289285948033248No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCA310.20289285948033248No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTC310.20289285948033248No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTC300.19634792852935404No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT290.18980299757837554No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC260.17016820472544014No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC260.17016820472544014No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAGT260.17016820472544014No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGCGGGGGTATCTAATCCCAGT250.16362327377446167No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC250.16362327377446167No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCA240.1570783428234832No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGCGGGGTATCTAATCCCAGTCAT230.15053341187250474No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATC230.15053341187250474No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCC210.1374435499705478No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT210.1374435499705478No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC210.1374435499705478No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCCAGTTCGAATCGCGATTGTCGG190.12435368806859087No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTC190.12435368806859087No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTTGGGGGTATCTAATCCCAGTCA190.12435368806859087No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCCA180.11780875711761242No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATC170.11126382616663394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTC108.3834527E-4295.02
GCGAAGT15150.0295.01
GAAGTTA15100.0294.023163
CGAAGTT15100.0294.023162
AAGTTAG15150.0293.05284
GTTAGAA15050.0293.039866
AGTTAGA15200.0292.08885
TTAGAAC15200.0289.17767
AGAACAG15750.0280.015879
TAGAACA16100.0272.096288
TTAGACC200.006673096147.57
GTTAGAC200.006673096147.56
AGACCAG200.006673096147.59
GGGTCTG750.059.00000425-29
CTGAGGC750.059.00000430-34
GGCGCTT750.059.00000465-69
TGTTCTG2750.059.00000475-79
CGCTTGT750.059.00000465-69
GCTGGCG750.059.00000460-64
GGATATA452.5465852E-1159.00000425-29
Produced by FastQC (version 0.11.5)