FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-58-4-1-3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-58-4-1-3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15436
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC274317.770147706659756No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG195012.632806426535373No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGAT13528.758745789064525No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC8385.428867582275201No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACC6644.301632547292044No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG4703.0448302669085257No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAAC4072.6366934438973826No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGA4032.6107799948173103No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCG2731.7685928997149518No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACC2591.677895827934698No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC2241.4511531484840632No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG2221.438196423944027No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC2161.399326250323918No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACC1811.1725835708732832No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1791.1596268463332469No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1450.9393625291526302No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT1450.9393625291526302No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1420.9199274423425758No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1270.8227520082923037No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTA1240.8033169214822493No Hit
AATCCTATTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT1080.699663125161959No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1060.6867064006219228No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1020.6607929515418502No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAAC1010.6543145892718321No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAAC980.6348795024617776No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC880.5700958797615963No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA790.511790619331433No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAA720.466442083441306No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT710.4599637211712879No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCG700.45348535890126973No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAA690.44700699663125165No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT650.42109354755117906No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT620.40165846074112466No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTA620.40165846074112466No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAA600.38870173620108833No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACC560.3627882871210158No Hit
AATCCTATTAGAACAGGCTCCTCTAGACATCGGGGTATCTAATCCCAGTA550.35630992485099766No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT530.3433532003109614No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACC500.323918113500907No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT500.323918113500907No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT490.3174397512308888No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC470.30448302669085253No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG470.30448302669085253No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTA460.29800466442083445No Hit
AATCCTATTAGAACAGGCTCCTCTGGGTCTCGTGGGGTATCTAATCCCAG450.29152630215081626No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAA430.27856957761078No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT410.26561285307074367No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC390.25265612853070746No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAAC380.2461777662606893No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC380.2461777662606893No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAG370.23969940399067116No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT370.23969940399067116No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATC360.233221041720653No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACC350.22674267945063487No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA330.21378595491059862No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTA330.21378595491059862No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAA320.20730759264058046No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGTCGGGGGTATCTAATCCCA300.19435086810054417No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA300.19435086810054417No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATC290.18787250583052606No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCGCTTCGGAAG270.17491578129048976No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC260.16843741902047163No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAA250.1619590567504535No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGCGGCACGCAGGGGTATCTAAT230.14900233221041723No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTAGGGGTATCTAATCCCA220.14252396994039906No Hit
AATCCTATTAGAACAGGCTCCTCTAGACCATTTCGGGGGGTATCTAATCC210.13604560767038093No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAA210.13604560767038093No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTA210.13604560767038093No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAG210.13604560767038093No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAAC200.1295672454003628No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC200.1295672454003628No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCA190.12308888313034465No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC190.12308888313034465No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACC180.1166105208603265No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTGGGGTATCTAATCCCAGTAACC170.11013215859030838No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG160.10365379632029023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAT108.3842577E-4294.999971
CCATTAG108.3842577E-4294.999974
AATCCTA15300.0294.035921
ATCCTAT15250.0294.032782
TCCTATT15250.0294.032783
CCTATTA15300.0293.071874
TATTAGA15200.0293.05926
CTATTAG15200.0293.05925
ATTAGAA15300.0289.215677
TTAGAAC15350.0288.27368
TAGAACA16300.0271.472389
ATCCATT150.0028225745196.666672
ATAGCGG150.0028225745196.66667295
TCCATTA150.0028225745196.666673
ATAGACC150.0028225745196.66667295
TTAGACA200.006673734147.499988
ATGGACG200.006673734147.49998295
GGCATAG900.059.000004125-129
GGTCCTT3100.059.00000445-49
TTTTAAG4550.059.00000455-59
Produced by FastQC (version 0.11.5)