FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-57-4-5-7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-57-4-5-7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15271
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC265617.39244319298016No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT13558.873027306659683No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC12568.224739702704472No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC7474.89162464802567No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCC7384.832689411302469No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT7384.832689411302469No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATC3642.3836029074716785No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC3382.2133455569379867No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATC3212.1020234431274964No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2651.735315303516469No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2281.4930259969877546No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2051.342413725361797No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAG2021.3227686464540633No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC1821.1918014537358392No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1751.1459629362844608No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1731.1328662170126382No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATC1621.0608342610176151No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCA1561.0215441032021477No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1541.0084473839303254No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTC1160.7596097177656997No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCA1120.7334162792220549No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCAT1090.7137712003143213No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1060.6941261214065877No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATC1020.6679326828629428No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC990.6482876039552092No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG970.6351908846833868No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT940.6155458057756532No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCC920.6024490865038308No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC770.5042236919651627No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC760.49767533232925154No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGTCAGGGGTATCTAATCCCAGT720.47148189378560673No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC700.4583851745137843No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA680.44528845524196187No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCAT670.43874009560605065No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCA640.4190950166983171No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC630.4125466570624059No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTC610.39944993779058346No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCAT590.38635321851876103No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC510.3339663414314714No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCATC500.3274179817955602No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCA490.320869622159649No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCAT490.320869622159649No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC460.3012245432519154No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCAT460.3012245432519154No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCC460.3012245432519154No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTTGCCAACAATCCTAGGGGTATCT450.2946761836160042No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTC440.28812782398009296No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG440.28812782398009296No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTC400.26193438543644815No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT360.23574094689280337No Hit
CCACGTCTAGAACAGGCTCCTCTAGCATCACGGGGGTATCTAATCCCAGT360.23574094689280337No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTC360.23574094689280337No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTC350.22919258725689215No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGCTCGGGGTATCTAATCCCAGTCA350.22919258725689215No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCC330.21609586798506975No Hit
CCACGTCTAGAACAGGCTCCTCTGGGTCTCGTGGGGTATCTAATCCCAGT320.20954750834915856No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCGCTTCGGAAGG310.20299914871324734No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA300.19645078907733612No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG300.19645078907733612No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC280.18335406980551372No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTCAGATGTGATAAGAGGGGTATC280.18335406980551372No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCC260.17025735053369132No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA260.17025735053369132No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTATCCGGGGTATCTAATCCCA250.1637089908977801No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG230.1506122716259577No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGTCTGGGCTCGGAGGGGTATCTAA220.14406391199004648No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC220.14406391199004648No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCAT210.1375155523541353No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCA210.1375155523541353No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC210.1375155523541353No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC200.13096719271822407No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC180.11787047344640168No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATCC180.11787047344640168No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAGT180.11787047344640168No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT170.11132211381049047No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCCGCGCGTCAGGGGTATCTAATCC160.10477375417457928No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGAGATCGGAAGGGCGGGGGTATC160.10477375417457928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA14900.0295.05
CGACTAA108.3834527E-4295.0295
CGTCTAG14950.0294.013374
CCACGTC15300.0293.07191
CACGTCT15250.0293.065582
ACGTCTA15150.0293.05283
TCTAGAA15050.0292.059786
CTAGAAC15050.0291.07977
TAGAACA15550.0279.823158
AGAACAG15550.0279.823159
CGTCTAA202.2383909E-5221.254
TAGAACG150.0028223048196.666668
ACGTCTG150.0028223048196.666663
GTCTGAA150.0028223048196.666665
AGAACGG150.0028223048196.666669
CCGCACA200.006673096147.5295
TCTGAAC200.006673096147.56
CGTCTGA200.006673096147.54
TCTAAAC352.0825877E-4126.4285666
GTCTAAA352.0825877E-4126.4285665
Produced by FastQC (version 0.11.5)