FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-56-6-5-8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-56-6-5-8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14733
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC836356.76372768614675No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC8065.470712007058983No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC3962.6878436163714112No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3902.6471187130930565No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC3722.524944003257992No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC3322.2534446480689607No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2711.839408131405688No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC2461.6697210344125433No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCA2381.6154211633747368No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1871.269259485508722No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1561.0588474852372225No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC1521.0316975497183194No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC1140.7737731622887396No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACTGCCAAGTCC960.6515984524536754No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT530.35973664562546664No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA450.3054367745876604No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC450.3054367745876604No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATC450.3054367745876604No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC430.2918618068282088No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC430.2918618068282088No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGGTATAAAGCACCGCCAAGTCC420.285074322948483No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT420.285074322948483No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC410.27828683906875723No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAATCACCGCCAGGTCC370.2511369035498541No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGAGATATGAAGCACCGCCAGGTCC370.2511369035498541No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTCAA360.2443494196701283No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAA350.23756193579040247No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT330.22398696803095092No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC310.21041200027149937No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT230.15611212923369305No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAA230.15611212923369305No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT230.15611212923369305No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC220.14932464535396728No Hit
CCACGTCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT210.1425371614742415No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA200.13574967759451573No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCA190.12896219371478992No Hit
CCACGTTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC180.12217470983506415No Hit
CCACGCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC180.12217470983506415No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC170.11538722595533836No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAAGTCC160.10859974207561257No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC160.10859974207561257No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC160.10859974207561257No Hit
CCACGTCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT150.10181225819588678No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTC150.10181225819588678No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAAT150.10181225819588678No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA108.3803665E-4295.03
ACGTCTA14450.0295.03
CGCCTAG108.3803665E-4295.04
CCACGGC108.3803665E-4295.01
CACGCCT108.3803665E-4295.02
GCCTAGA108.3803665E-4295.05
CACGTCT14450.0295.02
CCACGCC108.3803665E-4295.01
CCTAGAA108.3803665E-4295.06
ACAACGG108.3803665E-4295.0295
CGTCTAG14350.0295.04
CGTCTAA108.3803665E-4295.04
CACGGCT108.3803665E-4295.02
CCACGTC14500.0293.982761
GTCTAGA14400.0293.97575
CTAGAAC14600.0292.979467
TAGAACA14850.0288.047158
TCTAGAA14650.0287.95226
AGAACAG14950.0286.12049
ATATGAG150.002821268196.66667295
Produced by FastQC (version 0.11.5)