FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-55-5-4-1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-55-5-4-1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14756
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT401127.18216318785579No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG171811.64272160477094No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT11547.820547573868257No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT9126.180536730821361No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC5293.5849823800487934No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3082.0872865275142316No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG2311.5654648956356736No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2241.5180265654648957No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2221.5044727568446734No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCT1891.2808349146110056No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG1861.2605042016806722No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCC1771.1995120628896718No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC1691.1452968284087828No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1440.9758742206560044No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1400.9487666034155597No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1370.9284358904852263No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACC1360.9216589861751152No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG1200.813228517213337No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1090.7386825698021144No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTAC1030.6980211439414475No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTA930.6302521008403361No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT910.6166982922201139No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGT840.5692599620493358No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG830.5624830577392247No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACC770.5218216318785579No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT610.41339116291677963No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT520.35239902412577934No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT480.3252914068853348No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACC470.3185145025752236No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCC460.3117375982651125No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT450.30496069395500136No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTG450.30496069395500136No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT450.30496069395500136No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTA410.2778530767145568No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTAC410.2778530767145568No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT400.27107617240444565No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT400.27107617240444565No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTG370.25074545947411225No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCC370.25074545947411225No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG360.2439685551640011No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC360.2439685551640011No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCC360.2439685551640011No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTAC360.2439685551640011No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA350.23719165085388993No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT340.2304147465437788No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACCC330.22363784223366767No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGTG330.22363784223366767No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTACC320.21686093792355648No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT310.21008403361344538No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTG300.20330712930333425No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCT290.19653022499322312No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT290.19653022499322312No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACCC270.1829764163730008No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATCTA260.17619951206288967No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTG250.16942260775277854No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT240.1626457034426674No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT240.1626457034426674No Hit
GAGTGGTAGAACAGGCTCCTCTAGAACTACTGGGGTATCTAATCCCAGTG220.14909189482244511No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCC220.14909189482244511No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCCAG220.14909189482244511No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTGT210.14231499051233396No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCC210.14231499051233396No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTG210.14231499051233396No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCT210.14231499051233396No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT200.13553808620222282No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGTCGGGGGTATCTAATCCCAGTGT200.13553808620222282No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT190.1287611818921117No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTACC190.1287611818921117No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA190.1287611818921117No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA180.12198427758200055No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT170.1152073732718894No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT170.1152073732718894No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTGGGGTATCTAATCCCAGTGTACCC160.10843046896177824No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCAG150.10165356465166712No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT150.10165356465166712No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGCGGGGTATCTAATCCCAGTGTACC150.10165356465166712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGAA14500.0293.982765
GAGTGGT14700.0292.99321
AGTGGTA14700.0292.99322
TGGTAGA14650.0292.986334
GTGGTAG14700.0291.98983
GAACAGG14450.0291.93779
GTAGAAC15050.0283.23926
TAGAACA15250.0280.49187
AGAACAG15400.0275.844158
GGGTCTG1550.059.00000425-29
GTTGAGG4400.059.000004105-109
TATATTT950.059.00000495-99
GGCACCG1800.059.00000435-39
TTGTATA950.059.00000490-94
CCGCCAG4550.059.00000435-39
GCGAATG452.5465852E-1159.00000480-84
GTCTGAG1550.059.00000425-29
CTCTAGG7650.059.00000415-19
GGATATG4550.059.00000425-29
GCCAGGT4550.059.00000440-44
Produced by FastQC (version 0.11.5)