FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-54-5-4-1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-54-5-4-1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14677
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC434829.624582680384275No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG13208.993663555222458No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC11948.135177488587587No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCT9686.595353273829802No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTAC5473.7269196702323364No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3732.5413912924984667No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3312.2552292702868435No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3042.0712679702936567No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCC2211.5057573073516386No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA2181.4853171629079513No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACC1861.2672889555086189No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTA1831.2468488110649316No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTA1821.2400354295837024No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1611.0969544184778905No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTAC1491.015193840703141No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1491.015193840703141No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1310.8925529740410165No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1090.7426585814539757No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1030.7017782925666008No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT990.6745247666416843No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT880.5995775703481637No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG650.44286979627989376No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTA560.38154936294883146No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTAC550.3747359814676024No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC550.3747359814676024No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC530.3611092185051441No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT490.33385569258022757No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGT480.32704231109899845No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC460.31341554813654016No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC420.2861620222116236No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA420.2861620222116236No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGC400.2725352592491654No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGT370.2520951148054779No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA360.24528173332424882No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGT340.23165497036179056No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG330.22484158888056144No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTAC300.20440144443687405No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA290.19758806295564488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA290.19758806295564488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT290.19758806295564488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA290.19758806295564488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC290.19758806295564488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA280.19077468147441573No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG270.18396129999318664No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC270.18396129999318664No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG270.18396129999318664No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGT260.1771479185119575No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTAC260.1771479185119575No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT260.1771479185119575No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG240.16352115554949923No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTAC230.15670777406827008No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT230.15670777406827008No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT230.15670777406827008No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTAC230.15670777406827008No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTGT220.14989439258704093No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC220.14989439258704093No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTGTA210.1430810111058118No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC210.1430810111058118No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGT200.1362676296245827No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT200.1362676296245827No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC200.1362676296245827No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA190.12945424814335355No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTA190.12945424814335355No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGTCGGGGTATCTAAT190.12945424814335355No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC180.12264086666212441No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC170.11582748518089528No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG170.11582748518089528No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC170.11582748518089528No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACC170.11582748518089528No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG160.10901410369966613No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTG160.10901410369966613No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC150.10220072221843703No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC150.10220072221843703No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATACGAAGCACCGCCAGGTC150.10220072221843703No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC150.10220072221843703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAAT14750.0293.000031
TCAATCT14600.0292.979463
AATCTAG14550.0292.97255
CTCAATC14750.0292.000032
ATCTAGA14600.0291.969186
CAATCTA14600.0291.969184
TCTAGAA14550.0291.958777
CTAGAAC14650.0289.965888
TAGAACA15000.0282.216679
GAAAACC150.0028211703196.66667295
CAAGCGA200.0066704215147.5295
CGTTTGT452.5465852E-1159.00000465-69
TCCTTAG452.5465852E-1159.00000445-49
CTCCTCT14400.059.00000415-19
TAAGCGT452.5465852E-1159.00000460-64
GGCGAAT452.5465852E-1159.00000480-84
GAATGGT452.5465852E-1159.00000485-89
GGCTCCT14400.059.00000415-19
TTAAGCG452.5465852E-1159.00000460-64
TTTAGGG6100.059.000004115-119
Produced by FastQC (version 0.11.5)