FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-53-4-4-1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-53-4-4-1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14797
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG236816.0032439007907No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT225915.266608096235723No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT13248.947759681016421No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC8125.487598837602216No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT5823.9332297087247414No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG3232.1828749070757585No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCT3022.040954247482598No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC2741.8517267013583836No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG2591.7503548016489827No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCC2551.7233222950598093No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACC2121.4327228492261945No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG2071.3989322159897277No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1911.2908021896330337No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT1841.2434953031019802No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG1501.0137189970940055No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT1370.9258633506791918No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTAC1370.9258633506791918No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT1350.9123470973846051No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTA1340.9055889707373116No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGT1190.8042170710279111No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACC1070.7231195512603906No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT990.6690545380820436No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT870.5879570183145232No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACC820.5541663850780564No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT810.547408258430763No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTAC740.5001013718997094No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT720.48658511860512266No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTG720.48658511860512266No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT710.4798269919578293No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTG700.47306886531053594No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT690.4663107386632425No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCC670.4527944853686558No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT600.4054875988376022No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT600.4054875988376022No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTA580.39197134554301544No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGTG580.39197134554301544No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT580.39197134554301544No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC570.38521321889572213No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT560.37845509224842877No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCC560.37845509224842877No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG550.3716969656011354No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTAC530.35818071230654863No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT490.33114820571737513No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT490.33114820571737513No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCT480.32439007907008177No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA430.2905994458336149No Hit
GAGTGGTAGAACAGGCTCCTCTAGAACTACTGGGGTATCTAATCCCAGTG430.2905994458336149No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT420.28384131918632155No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACCC410.2770831925390282No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTG410.2770831925390282No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCC410.2770831925390282No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTG410.2770831925390282No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC380.25680881259714805No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCC380.25680881259714805No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTACC370.2500506859498547No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCT340.22977630600797458No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT330.22301817936068122No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCCAG330.22301817936068122No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTG320.21626005271338783No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGTCGGGGGTATCTAATCCCAGTGT310.20950192606609447No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGT300.2027437994188011No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACCC270.182469419476921No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTGT260.17571129282962764No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTACC260.17571129282962764No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT230.15543691288774752No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGCGGGGTATCTAATCCCAGTGTACC220.14867878624045414No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATCTA210.14192065959316078No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTGGGGTATCTAATCCCAGTGTACCC210.14192065959316078No Hit
GAGTGGTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAG190.12840440629857403No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG180.12164627965128066No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG180.12164627965128066No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCCTGGGGTATCTAATCCCAGTG170.11488815300398729No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTGATGGGGTATCTAATCCCAGTGT170.11488815300398729No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGTGT160.10813002635669391No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCCCGATCTCCTTGGGGTATCTAA160.10813002635669391No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT160.10813002635669391No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTAGGAGGGGTATCTA160.10813002635669391No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG150.10137189970940055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGAA14450.0295.05
GAGTGGT14600.0293.989721
TGGTAGA14550.0293.986274
GTGGTAG14650.0291.979523
GAACAGG14600.0289.948649
AGTGGTA14800.0289.020262
TAGAACA15000.0283.199987
AGAACAG15000.0283.199988
GTAGAAC15200.0279.47376
CTACAGG550.059.00000425-29
GTTGAGG2250.059.000004105-109
TATATTT550.059.00000495-99
TACAGGG550.059.00000425-29
CTAGGGG1800.059.00000420-24
GCAGTTT2250.059.00000485-89
AAGGTTT550.059.000004105-109
TATTTCT550.059.00000495-99
TGAGGTT2250.059.000004105-109
AGGCATA1100.059.000004120-124
ATATTTC550.059.00000495-99
Produced by FastQC (version 0.11.5)