FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-52-4-8-2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-52-4-8-2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14883
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC299720.137069139286435No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT12668.506349526305181No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT10967.364106698918229No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC8615.785123966942149No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT4953.325942350332594No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT4362.92951689847477No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4352.9227978230195526No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA3192.1433850702143387No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC3012.0224417120204263No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTG2521.6932070147147753No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC2421.6260162601626018No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2361.5857018074312972No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTT2181.464758449237385No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1781.1959954310286904No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1661.115366525566082No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1621.0884902237452125No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGT1511.0145803937378217No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT1350.9070751864543438No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1190.799569979170866No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1180.7928509037156486No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG1160.7794127528052139No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG1140.7659746018947793No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1050.705502922797823No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1010.6786266209769536No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG950.6383121682456494No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC810.5442451118726063No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGT810.5442451118726063No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG810.5442451118726063No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC790.5308069609621716No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG770.5173688100517368No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT760.5106497345965195No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTG690.46361620640999796No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGT670.45017805549956325No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT650.4367399045891286No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGT600.40314452731304173No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA510.34267284821608546No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA510.34267284821608546No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG500.3359537727608681No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT500.3359537727608681No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTG490.32923469730565075No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTT490.32923469730565075No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA480.32251562185043336No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC470.31579654639521604No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTA430.28892024457434656No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC430.28892024457434656No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT410.27548209366391185No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG410.27548209366391185No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT400.26876301820869447No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA400.26876301820869447No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG400.26876301820869447No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTG390.2620439427534771No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCACTCGATCTCATTGGGGTATCTA390.2620439427534771No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTGGGGTATCTAATC360.24188671638782502No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA360.24188671638782502No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC310.20829133911173825No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTT300.20157226365652087No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGACGGGGTATCTAATCCCAGTGT270.18141503729086877No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGGTATCTAATCCCAGTGTGT260.17469596183565142No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTGTTG260.17469596183565142No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTGGGGTATCTAATCCCAGTGTG250.16797688638043404No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAG250.16797688638043404No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTG250.16797688638043404No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGGTATCTAATCCCAG250.16797688638043404No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTG240.16125781092521668No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT240.16125781092521668No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTGT240.16125781092521668No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC230.15453873546999933No Hit
GCGAAGTTAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTGTG230.15453873546999933No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTGT230.15453873546999933No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATC220.14781966001478197No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTG220.14781966001478197No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG210.14110058455956462No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTT210.14110058455956462No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTCTGGGGTATCTAATCCCAGTGTG210.14110058455956462No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTT210.14110058455956462No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTT200.13438150910434724No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTG190.12766243364912988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA190.12766243364912988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTT190.12766243364912988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT180.12094335819391251No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAG180.12094335819391251No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT180.12094335819391251No Hit
GCGAAGTTAGAACAGGCTCCTCTTAGGGGTATCTAATCCCAGTGTGTTGC170.11422428273869516No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTGT170.11422428273869516No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTG160.10750520728347779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT14750.0295.01
GTTAGAA14550.0295.06
AAGTTAG14550.0295.04
CGAAGTT14750.0294.02
GAAGTTA14650.0293.993163
TTAGAAC14700.0291.98987
AGTTAGA14700.0291.98985
TAGAACA15400.0278.717568
AGAACAG15850.0270.80449
CACGAAA559.2285245E-6107.27272295
CGTTTGT150.004364410459.00000460-64
CAGCTCC150.004364410459.00000410-14
CGTCAGG150.004364410459.00000425-29
GTTTGTG150.004364410459.00000465-69
GGTTGGG303.299865E-759.00000425-29
CACATTC150.004364410459.000004185-189
AGCTCCT150.004364410459.00000410-14
TCTAGGC600.059.00000420-24
CAGCAAC150.004364410459.000004280-284
CTTTAAA150.004364410459.000004220-224
Produced by FastQC (version 0.11.5)