FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-51-6-7-5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-51-6-7-5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14182
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1082376.31504724298406No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT5513.8852065999153855No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT2241.5794669299111548No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT1611.1352418558736426No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1561.0999858976166972No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1290.9096037230291919No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT1080.7615286983500211No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT870.6134536736708504No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA650.4583274573402905No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGATCCT500.35255958256945424No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT410.2890988577069525No Hit
GAGACTTAGAACAGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT320.22563813284445072No Hit
GAGACTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT300.21153574954167254No Hit
GAGACTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT260.1833309829361162No Hit
GAGACTTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT260.1833309829361162No Hit
GAGACTTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT250.17627979128472712No Hit
GAGACTTAGAACGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT250.17627979128472712No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT210.14807502467917077No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCA200.14102383302778168No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTCTT200.14102383302778168No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT180.12692144972500352No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT180.12692144972500352No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT180.12692144972500352No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT170.11987025807361443No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT170.11987025807361443No Hit
GAGACTTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT170.11987025807361443No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCATCGCCAGGTCCT150.10576787477083627No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAA108.3770417E-4295.04
CTTAGAC157.0905025E-6295.05
CTAGCAA108.3770417E-4295.0295
AGACTTG108.3770417E-4295.02
AGACTTA13750.0295.02
CTTGAAC108.3770417E-4295.05
GACTTGA108.3770417E-4295.03
GAGACTT13900.0295.01
CTTAGAA13600.0293.915475
TTAGAAC13450.0293.903326
ACTTAGA13800.0292.862344
GAACAGG13500.0291.722239
TAGAACA13500.0290.629647
AGAACAG13950.0282.311838
GAAAAGG251.8177161E-7236.09
TTAGACA150.0028201512196.666676
TGAACAG150.0028201512196.666677
TTAGAAA255.4419022E-5177.06
GACTTAG26200.0154.81873
CAGACGA200.006668019147.5295
Produced by FastQC (version 0.11.5)