FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-50-6-5-8-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-50-6-5-8-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14107
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT726451.49216700928617No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT8455.9899340752817745No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCACT5523.912951017225491No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT3892.757496278443326No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT3722.6369887289997873No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT3372.3888849507336785No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT3282.3250868363223933No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1781.2617849294676402No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT1611.1412773800241016No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1410.9995037924434678No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT1350.9569717161692778No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATCAC1300.9215283192741193No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT1120.7939320904515488No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1110.7868434110725173No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1110.7868434110725173No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT980.6946905791451052No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT780.5529169915644715No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT740.5245622740483448No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT540.3827886864677111No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAGACACCGTCAGGTCCTT510.361522648330616No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAATCA430.30481321329836253No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAATC400.28354717516126743No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAATCA400.28354717516126743No Hit
CTAGGTAGAACAGGCTCCTCTCTCGGGGTATCTAATCCCAGTGTCAATCA380.2693698164032041No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT330.23392641950804563No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG330.23392641950804563No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTC320.22683774012901398No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTC310.2197490607499823No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT310.2197490607499823No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT300.21266038137095059No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT290.2055717019919189No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAGGTCCTT290.2055717019919189No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCTTT260.18430566385482383No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT250.17721698447579218No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT240.17012830509676047No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT190.13468490820160206No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCTT180.12759622882257035No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTCA180.12759622882257035No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGTC180.12759622882257035No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT170.12050754944353867No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAATC170.12050754944353867No Hit
CTAGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTCAA160.11341887006450699No Hit
CTAGGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT160.11341887006450699No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT150.10633019068547529No Hit
CTAGGTAGAACAGGCTCCTCTAGTGCCGACGGGGGTATCTAATCCCAGTG150.10633019068547529No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT150.10633019068547529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTA14100.0293.95391
AGGTAGA14050.0293.95023
TAGGTAG14050.0293.95022
GGTAGAA14000.0293.946444
GAACAGG13850.0293.935038
AACAGGC13800.0292.86239
TAGAACA14300.0286.748266
AGAACAG14300.0286.748267
GTAGAAC14450.0284.79245
ACGAGAA450.00986721465.55556295
CTTTTGA303.2974458E-759.00000445-49
CTCTGGC600.059.00000475-79
CGTTTCA150.00436349659.000004215-219
CGCTGCA150.00436349659.000004175-179
TAGAAAT150.00436349659.000004275-279
GTTGATT8600.059.00000490-94
AGGGGGT150.00436349659.00000420-24
TTGTTGA8500.059.00000490-94
GGATGCT150.00436349659.00000485-89
GTCACTC150.00436349659.000004145-149
Produced by FastQC (version 0.11.5)