FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-5-4-5-7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-5-4-5-7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT191816.898678414096917No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC151413.33920704845815No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT9988.79295154185022No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA6635.8414096916299565No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT5464.81057268722467No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC2852.511013215859031No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC2592.2819383259911894No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCC2101.8502202643171806No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGC2091.8414096916299558No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1571.3832599118942732No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1521.3392070484581498No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA1521.3392070484581498No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC1411.2422907488986783No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1381.2158590308370043No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT1361.198237885462555No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCATC1251.1013215859030838No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT1231.0837004405286343No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG1221.0748898678414098No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCA950.8370044052863436No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCC910.801762114537445No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTC860.7577092511013216No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT830.7312775330396476No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT770.6784140969162996No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCC750.6607929515418502No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCA580.5110132158590308No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCAT580.5110132158590308No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT570.5022026431718062No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCA570.5022026431718062No Hit
TGCTTATAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTCAT510.44933920704845814No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT460.4052863436123348No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCA450.39647577092511016No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCAT440.3876651982378855No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC430.3788546255506608No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT420.3700440528634361No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC410.3612334801762115No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT380.33480176211453744No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCCA380.33480176211453744No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT380.33480176211453744No Hit
TGCTTATAGAACAGGCTCCTCTAGAACTACCGGGGTATCTAATCCCAGTC380.33480176211453744No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG370.32599118942731276No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC370.32599118942731276No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGCAGTCAGGGGTATCTAATCCCA360.31718061674008813No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCCA350.30837004405286345No Hit
TGCTTATAGAACAGGCTCCTCTAGCACGGGGTATCTAATCCCAGTCATCC350.30837004405286345No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTC340.29955947136563876No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGCGGGGTATCTAATCCCAGTC330.29074889867841414No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTAATGTGTATAGTGGGGTATCTAA330.29074889867841414No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT330.29074889867841414No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT310.2731277533039647No Hit
TGCTTATAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTCATC300.2643171806167401No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT290.2555066079295154No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG290.2555066079295154No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCA270.2378854625550661No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGCAGGGGTATCTAATCCCAGTCA270.2378854625550661No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTC260.2290748898678414No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCAGT260.2290748898678414No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT250.22026431718061676No Hit
TGCTTATAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAG240.21145374449339205No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTC230.2026431718061674No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCA210.18502202643171806No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTCAGGGGTATCTAATCCCAGTCA210.18502202643171806No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGT210.18502202643171806No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT200.1762114537444934No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCA200.1762114537444934No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCTCGTGGGCTCGGGGTATCTAATC170.14977973568281938No Hit
TGCTTATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCATCCAG170.14977973568281938No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCA170.14977973568281938No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT160.14096916299559473No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT150.13215859030837004No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT140.12334801762114538No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCA140.12334801762114538No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAGTC130.1145374449339207No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT120.10572687224669602No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCATCC120.10572687224669602No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCATC120.10572687224669602No Hit
TGCTTATAGAACAGGCTCCTCTAGACTGGGGTATCTAATCCCAGTCATCC120.10572687224669602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG11300.0295.03
GCTTATA11300.0295.02
TGCTTAT11400.0293.706121
TTATAGA11400.0292.412264
GAACAGG11250.0292.377789
TATAGAA11650.0282.339055
AGAACAG11700.0281.132488
ATAGAAC11900.0276.407566
TAGAACA11950.0275.251077
CAGATTA150.002812793196.66667295
CGCCACA200.006650665147.49998295
CTACAGG452.5465852E-1159.00000425-29
TGTTGCT452.5465852E-1159.00000460-64
GGGTCTG452.5465852E-1159.00000425-29
GGGTTTT452.5465852E-1159.00000450-54
GTTGAGG1800.059.000004105-109
CTGAGGC452.5465852E-1159.00000430-34
GGCGCTT452.5465852E-1159.00000465-69
TTTCTAT452.5465852E-1159.000004100-104
GTTCCCT452.5465852E-1159.00000475-79
Produced by FastQC (version 0.11.5)