FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-49-5-2-6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-49-5-2-6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14386
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC511135.52759627415543No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC169711.796190740998194No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT6524.532184067843737No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT5433.7745029890171No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4913.4130404559988876No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC3752.606700959265953No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG3642.530237731127485No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT2551.7725566523008482No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC2501.7378006395106351No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC2051.424996524398721No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1651.1469484220770194No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTG1300.9036563325455305No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCC1250.8689003197553176No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1130.7854858890588071No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1100.7646322813846795No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1080.7507298762685944No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1010.7020714583622967No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGG910.6325594327818712No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG800.5560962046434033No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGC770.5352425969692757No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT710.49353538162102045No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC700.48658417906297785No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG680.4726817739468928No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC660.4587793688308077No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT620.4309745585986376No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT540.3753649381342972No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC470.32670652022799945No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC440.3058529125538718No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT390.27109689976365914No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTT380.26414569720561654No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTT370.25719449464757405No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTT360.2502432920895315No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTTGT340.2363408869734464No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA330.22938968441540386No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTG330.22938968441540386No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT330.22938968441540386No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTG320.22243848185736131No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCA310.2154872792993188No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGG310.2154872792993188No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTGT280.19463367162519116No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC270.1876824690671486No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTGT270.1876824690671486No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTT270.1876824690671486No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTT250.17378006395106352No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGG240.166828861393021No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTTGT240.166828861393021No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC230.15987765883497845No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG230.15987765883497845No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGT230.15987765883497845No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTG220.1529264562769359No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG210.14597525371889336No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCACGTCGGGGTATCTAATCCCAGT200.13902405116085081No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAATC190.13207284860280827No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCA190.13207284860280827No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGCTCTTTCGATCTCGGGGTATCTA180.12512164604476575No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT180.12512164604476575No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTGGGGTATCTAATCCCAGTT170.1181704434867232No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTCTGAGGCACCGCCAAGTCC170.1181704434867232No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC170.1181704434867232No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC170.1181704434867232No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT160.11121924092868066No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTT150.10426803837063811No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCCA150.10426803837063811No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTT150.10426803837063811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT14300.0295.01
CGAAGTT14250.0293.96492
GTTAGAA14100.0293.95396
AAGTTAG14100.0293.95394
AGTTAGA14100.0293.95395
TTAGAAC14250.0291.894747
GAAGTTA14300.0290.874153
AGAACAG14850.0278.114479
TAGAACA15000.0276.316658
GAAGTTG150.0028205675196.666673
CAGAGAA600.00177256373.75295
GCCCTCG150.004363811659.00000425-29
CTTTTGA150.004363811659.00000445-49
TCCTCTA14050.059.00000415-19
TTTTGAG150.004363811659.00000450-54
TTGTTGG150.004363811659.00000490-94
ACCGGGG150.004363811659.00000430-34
GGTTGTC303.2982825E-759.000004205-209
CTTCAAA150.004363811659.000004220-224
GGGTTTA303.2982825E-759.000004110-114
Produced by FastQC (version 0.11.5)