FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-48-5-3-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-48-5-3-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14017
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC273719.526289505600342No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG180112.848683741171435No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC10787.690661339801669No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC6744.808446885924235No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCA5864.180637796960833No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTC3292.34714988941999No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAAT2842.0261111507455234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC2391.7050724120710565No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC2271.6194620817578653No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTC2061.4696440037097809No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1841.3126917314689306No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1721.2270814011557396No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAA1691.2056788185774416No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG1601.1414710708425484No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1481.0558607405293572No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA1340.9559820218306342No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCA1340.9559820218306342No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1330.9488478276378683No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1320.9417136334451024No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1310.9345794392523363No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1140.8132981379753157No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1090.777627167011486No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG1060.7562245844331883No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTC970.692016836698295No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA960.684882642505529No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA830.592138117999572No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC820.5850039238068061No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC810.57786972961404No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTC770.5493329528429765No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT750.5350645644574445No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG750.5350645644574445No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG690.492259399300849No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA670.4779910109153171No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTCA630.4494542341442534No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTCA610.43518584575872155No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAA600.42805165156595565No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTCA590.42091745737318964No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG560.39951487479489195No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGT510.3638439038310623No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC470.33530712705999854No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC410.29250196190340305No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC390.27823357351787115No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG390.27823357351787115No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAG390.27823357351787115No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC390.27823357351787115No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC370.2639651851323393No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTCA360.25683099093957334No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGT340.2425626025540415No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGCGGGGTATCTAATCCCAGTGTC330.2354284083612756No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC320.22829421416850967No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTG300.21402582578297782No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGT280.19975743739744597No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTCA280.19975743739744597No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAG270.19262324320468005No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT270.19262324320468005No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC270.19262324320468005No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG260.1854890490119141No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG230.16408646643361632No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTGT230.16408646643361632No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT220.1569522722408504No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGCGGGCTCGGGGTATCTAA220.1569522722408504No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGT220.1569522722408504No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC210.14981807804808447No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGT210.14981807804808447No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT210.14981807804808447No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT200.14268388385531855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT200.14268388385531855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT200.14268388385531855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAG190.1355496896625526No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCA190.1355496896625526No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTC180.12841549546978667No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTCGGGGTATCTAATCCCAGTGT180.12841549546978667No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC180.12841549546978667No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAG170.12128130127702075No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG170.12128130127702075No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAG170.12128130127702075No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTGGGGTATCTAATCCCAGTGTC170.12128130127702075No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT160.11414710708425484No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGTCGGGGTATCTAATCCCA160.11414710708425484No Hit
TTCTCAGCTAGAACAGGCTCCTCTATGTTCGTGGGGGTATCTAATCCCAG150.10701291289148891No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGGGGGTATCTAATCCCAGT150.10701291289148891No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC150.10701291289148891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCAG13900.0295.01
CTCAGCT13900.0295.03
TCAGCTA13850.0292.870034
TCTCAGC14050.0291.850522
CAGCTAG13850.0291.805055
GCTAGAA13850.0291.805057
AGCTAGA13900.0290.755376
CTAGAAC14050.0285.55168
TAGAACA14400.0278.61119
TCAGCTG150.0028198317196.666674
AGCTGAA150.0028198317196.666676
GCTGAAC200.006667265147.57
CGTTTGT150.004363367359.00000465-69
CTCTGGC257.757957E-659.00000480-84
CGCTGCG150.004363367359.000004180-184
TAGCGCG150.004363367359.00000420-24
AGGGGGG150.004363367359.00000425-29
ACCGGGG257.757957E-659.00000430-34
GGATGGA150.004363367359.00000430-34
GGGTCTG501.8189894E-1259.00000425-29
Produced by FastQC (version 0.11.5)