FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-47-4-2-6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-47-4-2-6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14306
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC284119.858800503285334No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT12949.045155878652315No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT12038.409059136026842No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC9836.871242835174053No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG5884.110163567733818No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4182.9218509716202994No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3032.117992450719978No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2962.0690619320564796No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2731.9082902278764156No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCC2531.7684887459807075No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGC2291.6007269677058578No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTG2251.572766671326716No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2081.4539354117153642No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGG1791.2512232629665876No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTT1691.1813225220187333No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTG1441.0065706696490984No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG1441.0065706696490984No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1420.9925905214595274No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1390.9716202991751712No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1270.8877394100377464No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTG1240.8667691877533902No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1200.8388088913742486No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1160.810848594995107No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTGT900.6291066685306864No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC880.6151265203411156No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGG880.6151265203411156No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC850.5941562980567594No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC630.4403746679714805No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTT610.4263945197819097No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG610.4263945197819097No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTG600.4194044456871243No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTTG600.4194044456871243No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGC590.41241437159233885No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC540.37746400111841183No Hit
CGATTCCTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTT530.37047392702362647No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGG520.36348385292884106No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG500.3495037047392702No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTT500.3495037047392702No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTTG470.32853348245491404No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA400.2796029637914162No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGT400.2796029637914162No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGG400.2796029637914162No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTTG380.26562281560184536No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG370.25863274150705995No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC360.2516426674122746No Hit
CGATTCCTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGTT330.23067244512791837No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTTGT320.22368237103313296No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTGT310.21669229693834755No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT300.20970222284356216No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTT300.20970222284356216No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTTGT280.19572207465399133No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTTGT280.19572207465399133No Hit
CGATTCCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA270.18873200055920591No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGCGGGGTATCTAATCCCAGTTG270.18873200055920591No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAG260.18174192646442053No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT250.1747518523696351No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTGG250.1747518523696351No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTTGT240.1677617782748497No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT240.1677617782748497No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGT230.1607717041800643No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA220.1537816300852789No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGTGGGGTATCTAATCCCAGTTG220.1537816300852789No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGT210.14679155599049348No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGG210.14679155599049348No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC210.14679155599049348No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTT200.1398014818957081No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC200.1398014818957081No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC190.13281140780092268No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT190.13281140780092268No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT190.13281140780092268No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTTGTG180.1258213337061373No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC170.11883125961135188No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT170.11883125961135188No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTAGTGGGGTATCTAATCCCAGTT170.11883125961135188No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTG160.11184118551656648No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTTG160.11184118551656648No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTT150.10485111142178108No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC150.10485111142178108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA14150.0295.05
TTCCTAG14150.0295.04
GATTCCT14200.0295.02
ATTCCTA14150.0295.03
CAGCGCG108.3779736E-4295.0295
CGATTCC14300.0293.968541
CCTAGAA14150.0293.95766
CTAGAAC14150.0291.87287
TAGAACA15000.0274.358
AGAACAG15100.0272.533149
CGAGAGA200.0066687567147.5295
AGGGGGT501.8189894E-1259.00000420-24
GTTGAGG3350.059.000004105-109
GAGCGGT257.759656E-659.00000485-89
TCCTTAG257.759656E-659.00000445-49
GTGTTCT3800.059.00000475-79
TAGTGTT3800.059.00000470-74
CTGGGGT501.8189894E-1259.00000430-34
CTCTTTG257.759656E-659.000004190-194
TGTTCTG3800.059.00000475-79
Produced by FastQC (version 0.11.5)