FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-46-6-4-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-46-6-4-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC453233.48603517068125No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC143810.625092359982267No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC13489.960100487660705No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC7485.526821338850303No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC6384.714053494901729No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG5844.315058371508792No Hit
TTCTCAGCTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCT4573.376680951677257No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC3552.6230234963794885No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC3152.327471553125462No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3042.2461947687306045No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC2671.9728092212206296No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTCA1811.3373725432244716No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTC1711.263484557410965No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCA1611.1895965715974584No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATTGAGACTGTCTCTTATACACAT1361.0048766070636914No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC1120.8275454411112753No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG910.6723806709029112No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTC770.5689374907640018No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA750.5541598936013004No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAA610.45071671346239106No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC610.45071671346239106No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAA590.4359391162996897No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCA580.428550317718339No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG390.28816314467267623No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAAT230.16994236737106547No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC150.11083197872026009No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC150.11083197872026009No Hit
TTCTCAGCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC140.10344318013890941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAA13100.0295.07
TCAGCTA13250.0295.04
TTCTCAG13450.0295.01
CTCAGCT13250.0295.03
TCTCAGT108.372762E-4295.02
TCTCAGC13300.0295.02
CAGCTAG13350.0292.790255
CTAGAAC13100.0292.748088
AGCTAGA13400.0291.697756
TAGAACA13250.0290.547189
CTAGAAA150.0028187148196.666668
GCTAGAC255.436354E-5177.07
ACCGCAA200.00666463147.5295
AACAGCG200.00666463147.5295
ACGACAG301.1242762E-4147.49998295
TCCTCTA12300.059.00000415-19
AACCGCC900.059.00000435-39
GGCACCG1800.059.00000435-39
CTTTAGG900.059.000004115-119
CTCCTCT13250.059.00000415-19
Produced by FastQC (version 0.11.5)