FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-45-4-4-1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-45-4-4-1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13752
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC250218.19371727748691No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG168812.27457824316463No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCT13559.853112274578244No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTAC7595.519197207678883No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC6254.544793484584061No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG5594.064863292611983No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACC2641.9197207678883073No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCC2581.8760907504363002No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2481.8033740546829553No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTA2461.7888307155322862No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTA2341.7015706806282722No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTAC1941.4107038976148925No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC1861.3525305410122164No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC1831.330715532286213No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1370.9962187318208261No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1210.879872018615474No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT1080.7853403141361256No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC860.6253635834787667No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT800.5817335660267597No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG770.5599185573007562No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTAC770.5599185573007562No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC700.5090168702734148No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTA670.4872018615474113No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC660.4799301919720768No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG650.4726585223967423No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGT620.4508435136707388No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT570.41448516579406636No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGT560.4072134962187318No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTAC550.39994182664339734No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGT550.39994182664339734No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA550.39994182664339734No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT540.3926701570680628No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT510.37085514834205935No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC510.37085514834205935No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGC510.37085514834205935No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTGT470.34176847004072136No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA470.34176847004072136No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG440.31995346131471786No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTAC440.31995346131471786No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA430.3126817917393834No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA390.28359511343804533No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG390.28359511343804533No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG380.2763234438627109No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA370.26905177428737637No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACC370.26905177428737637No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA370.26905177428737637No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA370.26905177428737637No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG370.26905177428737637No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA350.2545084351367074No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGTCGGGGTATCTAAT350.2545084351367074No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTAC340.24723676556137286No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC340.24723676556137286No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTAC340.24723676556137286No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTG340.24723676556137286No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT330.2399650959860384No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC320.2326934264107039No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG290.2108784176847004No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGT280.2036067481093659No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC270.1963350785340314No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTA260.18906340895869692No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG250.1817917393833624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGT250.1817917393833624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTGTA250.1817917393833624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC250.1817917393833624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGGGGGTATCTAATCCCAG230.16724840023269344No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC230.16724840023269344No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACGGGGTATCTAATCCCAGTGTA230.16724840023269344No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACC200.14543339150668994No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC200.14543339150668994No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG190.13816172193135545No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGT180.13089005235602094No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTA170.12361838278068643No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC160.11634671320535195No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGATTCTTGGGGTATCTAATCCC160.11634671320535195No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC150.10907504363001745No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTA150.10907504363001745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAAT13600.0295.000031
TCAATCT13500.0295.03
TCTGAAC157.086119E-6294.999977
CAATCTA13400.0293.899264
CTCAATC13700.0292.84672
AATCTAG13350.0292.790255
TCTAGAA13250.0292.77367
ATCTAGA13500.0289.537056
CTAGAAC13500.0288.444468
TAGAACA14000.0278.142859
TGATGAA150.0028192836196.66666295
CATCTAG150.0028192836196.666664
ATCTGAA255.43855E-5177.06
CTCATCT200.006665971147.52
CAATCTG301.1247297E-4147.499984
AATCTGA352.0781426E-4126.428585
CTGAACA403.5357653E-4110.6258
TGAACAG455.6485104E-498.333339
CTCTGGC452.5465852E-1159.00000480-84
GGGTCTG452.5465852E-1159.00000425-29
Produced by FastQC (version 0.11.5)