FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-44-5-3-8-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-44-5-3-8-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13470
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC347325.783221974758725No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT163212.115812917594655No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC8936.629547141796585No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCA7175.32293986636971No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAA3852.858203414996288No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT2641.9599109131403119No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC2521.8708240534521157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2491.8485523385300666No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2271.6852264291017074No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCA2211.6406829992576095No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAA2031.5070527097253155No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1961.4550853749072012No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATC1921.4253897550111359No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1901.4105419450631032No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAAT1721.276911655530809No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT1661.2323682256867112No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1511.1210096510764662No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTCA1180.8760207869339274No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1160.8611729769858946No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1150.8537490720118782No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1080.8017817371937639No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC970.7201187824795843No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTCA910.6755753526354862No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG860.6384558277654045No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTCA740.5493689680772086No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTCA650.48255382331106156No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTCAA650.48255382331106156No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTCA620.4602821083890127No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTG620.4602821083890127No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG530.39346696362286565No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC520.3860430586488493No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT520.3860430586488493No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG440.3266518188567187No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG430.3192279138827023No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGT420.311804008908686No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG400.2969561989606533No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTC400.2969561989606533No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTC390.289532293986637No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC360.26726057906458794No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA340.2524127691165553No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC340.2524127691165553No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGT330.24498886414253898No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA320.23756495916852266No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTGTC310.23014105419450634No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTCAA280.20786933927245732No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT260.19302152932442465No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA260.19302152932442465No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC240.17817371937639198No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCAG240.17817371937639198No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTCAA230.17074981440237563No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA230.17074981440237563No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC220.16332590942835934No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGATCAGGGGTATCTAATCCCAGT220.16332590942835934No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTCAA200.14847809948032664No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAG200.14847809948032664No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC200.14847809948032664No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG190.14105419450631032No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA180.13363028953229397No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGT180.13363028953229397No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTC180.13363028953229397No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTC180.13363028953229397No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTTGGGGGTATCTAATCCCAGTGT180.13363028953229397No Hit
GCGAAGTTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTG180.13363028953229397No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCTCGTGGGGTATCTAATCCCAGT170.12620638455827765No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTGTC170.12620638455827765No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGT150.11135857461024498No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTC150.11135857461024498No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT150.11135857461024498No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTCA150.11135857461024498No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATC140.10393466963622866No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT140.10393466963622866No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT140.10393466963622866No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC140.10393466963622866No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCCA140.10393466963622866No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTTG108.372416E-4295.03
CGAAGTT13200.0295.02
GTTAGAC108.372416E-4295.06
AAGTTAG13150.0293.878334
AGTTAGA13150.0293.878335
GCGAAGT13350.0292.790251
GAAGTTA13200.0292.765143
GTTAGAA13050.0292.739476
TTAGAAC13200.0289.412877
TAGAACA13550.0284.114388
AGAACAG13700.0282.08039
AGACAGG200.0066643558147.59
CTCCTCT13250.059.00000415-19
GTTCCCT900.059.00000475-79
AGGGCTA3600.059.000004115-119
GGTTTAG3750.059.000004110-114
TAGTTCC900.059.00000470-74
CTAGGCA900.059.000004120-124
TTTTAAG4900.059.00000455-59
TTCCCTG900.059.00000475-79
Produced by FastQC (version 0.11.5)