FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-43-6-3-8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-43-6-3-8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13293
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT767657.74467764989092No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT10287.733393515384036No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT8776.597457308357782No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA3662.7533288196795307No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2431.8280297901150981No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT1701.2788685774467765No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT1681.263823064770932No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCAC1421.0682313999849544No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT1280.9629128112540434No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1180.8876852478748214No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAAT1080.8124576844955992No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1050.7898894154818324No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGATATAAAGTACCGCCAAGTCCT1040.7823666591439102No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTCAA1020.7673211464680658No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG1000.7522756337922215No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT890.6695253140750771No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAAT850.6394342887233883No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCAGATGTGTATAAAGACGGGGTAT700.526592943654555No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA670.5040246746407884No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAGGTCCT570.4287971112615662No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT480.3610923042202663No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG460.34604679154442186No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTC420.315955766192733No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT290.21815993379974424No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCCT190.14293237042052206No Hit
GAGTGGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT180.13540961408259986No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCCT170.12788685774467765No Hit
GAGTGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT160.12036410140675542No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT150.11284134506883321No Hit
GAGTGGTAGAAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT140.105318588730911No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACC108.371005E-4295.06
GGTGAAC108.371005E-4295.05
GAGTGGT13100.0295.01
AGTGGTG108.371005E-4295.02
CGAACGA108.371005E-4295.0295
GTGAACA108.371005E-4295.06
GACCAGG108.371005E-4295.09
GACAGGC157.080267E-6294.999979
AGTGGTA13050.0293.869752
GGTAGAA12800.0293.847665
TGGTAGA13100.0292.748084
GAACAGG12800.0289.238289
GTGGTAG13350.0287.265933
GTAGAAC12950.0287.027046
TAGAACA13250.0278.301887
AGAACAG13350.0277.32218
AGACAGG202.231747E-5221.258
GGTAGAC353.76167E-9210.714285
CCAACTA150.002818125196.66666295
GTGGTGA150.002818125196.666663
Produced by FastQC (version 0.11.5)