FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-42-4-5-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-42-4-5-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC211515.638864241348713No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG184113.612836438923395No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG12659.35374149659864No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATC7995.90801538006507No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC5454.029872818692694No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG5033.7193138124815146No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3592.6545400769003256No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT2912.1517302573203194No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2591.9151138716356109No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA2531.870748299319728No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2251.6637089618456078No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1791.3235729074238392No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATC1781.316178645371192No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCC1691.2496302868973677No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCAT1651.220053238686779No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC1451.0721679976338363No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCAT1411.0425909494232475No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCA1240.9168884945282461No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC1180.8725229222123632No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCAT1170.865128660159716No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA1040.7690032534753032No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATC960.709849157054126No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTC880.6506950606329488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT880.6506950606329488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT880.6506950606329488No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGCGTCAGGGGTATCTAATCCCA850.6285122744750073No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT830.6137237503697132No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG790.5841467021591245No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA740.5471753918958887No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATC720.5323868677905945No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC720.5323868677905945No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCA690.5102040816326531No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC670.4954155575273588No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC640.47323277136941727No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC630.4658385093167702No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC610.45104998521147593No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATC560.4140786749482402No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTC500.3697131026323573No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCA450.33274179236912155No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACCTCGATCTCCTTGGGGTATCT410.30316474415853295No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC400.29577048210588586No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA390.2883762200532387No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGTGGGGTATCTAATCCCAGTC380.28098195800059156No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGT380.28098195800059156No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGT340.25140490979000296No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCA330.2440106477373558No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATC330.2440106477373558No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCACGTCGGGGTATCTAATCCCAG320.23661638568470864No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCAGGGGTATCTAATCCCAGTC320.23661638568470864No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGAGGGGTATC260.1922508133688258No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTC240.1774622892635315No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCC220.1626737651582372No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC220.1626737651582372No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT200.14788524105294293No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG190.14049097900029578No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC180.13309671694764863No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCACAGGGGTATCTAATCCCAGT170.12570245489500148No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCC170.12570245489500148No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTC170.12570245489500148No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCC170.12570245489500148No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGT160.11830819284235432No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAG150.11091393078970718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAG13300.0295.05
TCTCAAT13400.0293.899261
TCTAGAA13200.0293.88267
TCAATCT13450.0292.80673
CTCAATC13500.0291.722232
CAATCTA13450.0291.710054
ATCTAGA13450.0290.613376
CTAGAAC13450.0288.420078
TAGAACA13900.0279.082739
GGGTCTG452.5465852E-1159.00000425-29
GAGCGGT452.5465852E-1159.00000485-89
TCTAGGG1800.059.00000420-24
GGCGCTT452.5465852E-1159.00000465-69
CTCCTCT13250.059.00000415-19
GCGGTTT452.5465852E-1159.00000485-89
TTCCCTG452.5465852E-1159.00000475-79
GTGGGTC452.5465852E-1159.00000425-29
AGCGGTT452.5465852E-1159.00000485-89
GCGCTTG452.5465852E-1159.00000465-69
GGCTCCT13200.059.00000415-19
Produced by FastQC (version 0.11.5)