FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-41-4-3-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-41-4-3-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13503
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG213615.81870695401022No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC141510.479152780863512No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC140810.427312449085388No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCA6664.93223728060431No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAAT3882.873435532844553No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTC3722.7549433459231283No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC3572.6438569206842923No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTC2752.03658446271199No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2331.7255424720432497No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG2171.607050285121825No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2051.518181144930756No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAA1971.4589350514700439No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA1861.3774716729615641No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1801.3330371028660297No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1641.214544915944605No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1531.1330815374361254No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCA1481.09605272902318No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1421.0516181589276457No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC1310.970154780419166No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG1310.970154780419166No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC1310.970154780419166No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1300.9627490187365771No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTC1070.7924165000370288No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1050.7776049766718507No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1040.7701992149892616No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT930.6887358364807821No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGT810.5998666962897135No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG810.5998666962897135No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTC790.5850551729245353No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG790.5850551729245353No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA760.5628378878767681No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTCA730.5406206028290009No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTCA640.4739687476856995No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTCA610.45175146263793226No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAA600.4443457009553432No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC570.4221284159075761No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC500.3702880841294527No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG480.3554765607642746No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGT480.3554765607642746No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGCGGGGTATCTAATCCCAGTGTC480.3554765607642746No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC440.3258535140339184No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTCA430.3184477523513293No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTCA410.3036362289861512No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGT400.2962304673035622No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG400.2962304673035622No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC390.2888247056209731No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAG360.26660742057320597No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAG360.26660742057320597No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT360.26660742057320597No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTG360.26660742057320597No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCA350.2592016588906169No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC340.25179589720802786No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGT340.25179589720802786No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT330.2443901355254388No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC320.23698437384284976No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT310.22957861216026065No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTCGGGGTATCTAATCCCAGTGT270.19995556542990447No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGTCAGGGGTATCTAATCCCAG270.19995556542990447No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAG270.19995556542990447No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG260.1925498037473154No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT250.18514404206472634No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTGT250.18514404206472634No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT250.18514404206472634No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTC240.1777382803821373No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC230.17033251869954827No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG230.17033251869954827No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGT230.17033251869954827No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC220.1629267570169592No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGT220.1629267570169592No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA200.1481152336517811No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAG200.1481152336517811No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAG200.1481152336517811No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTCAA190.14070947196919203No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT190.14070947196919203No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTGGGGTATCTAATCCCAGTGTC190.14070947196919203No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGTGGGGTATCTAATCCCAGTG180.13330371028660298No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGGGGGTATCTAATCCCAGT180.13330371028660298No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAG180.13330371028660298No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTG180.13330371028660298No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTG170.12589794860401393No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC170.12589794860401393No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC170.12589794860401393No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGCTAGGGGTATCTAATCCCAG160.11849218692142488No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGT160.11849218692142488No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAAT160.11849218692142488No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCC140.10368066355624676No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGCGGGCTCGGGGTATCTAA140.10368066355624676No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGTCGGGGTATCTAATCCCA140.10368066355624676No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCAG108.372762E-4295.04
TTCTCAG13200.0295.01
CTCGCTA108.372762E-4295.03
TTCTCGC157.0832466E-6294.999971
TCTCAGC13200.0293.88262
CTCAGCT13100.0293.874053
AGCTAGA12900.0292.713176
CTAGAAC13000.0291.596168
GCTAGAA13050.0290.47897
CAGCTAG13000.0290.461555
TCAGCTA13100.0289.37024
TAGAACA13650.0275.549449
TCAGCTG202.2326845E-5221.254
CGCTAGA200.00666463147.55
TCTCGCT200.00666463147.52
TCGCTAG200.00666463147.54
GCTGAAC301.1242762E-4147.499987
CAGCTGA301.1242762E-4147.499985
AGCTGAA403.5343453E-4110.6256
TGAACAG508.582832E-488.59
Produced by FastQC (version 0.11.5)