FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-40-6-5-7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-40-6-5-7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13275
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC618546.591337099811675No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC11048.31638418079096No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC7465.619585687382298No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC4053.050847457627119No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3292.4783427495291903No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG3182.3954802259887007No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC2752.0715630885122414No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2742.0640301318267418No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC1130.8512241054613935No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA1110.8361581920903955No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC920.6930320150659134No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC730.5499058380414312No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC660.4971751412429379No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAGGTC620.4670433145009416No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC540.4067796610169492No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC500.3766478342749529No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC460.3465160075329567No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC400.3013182674199623No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC390.29378531073446323No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG390.29378531073446323No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTC380.2862523540489642No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC370.2787193973634651No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTCA360.2711864406779661No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTCATC320.24105461393596986No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC320.24105461393596986No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATC270.2033898305084746No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTC260.1958568738229755No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC250.18832391713747645No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC240.1807909604519774No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC210.1581920903954802No Hit
AATCCTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC190.1431261770244821No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT180.13559322033898305No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCC180.13559322033898305No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATC170.128060263653484No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC170.128060263653484No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC170.128060263653484No Hit
AATCCTATTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTC170.128060263653484No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGTCTCGTGGGGTATCTAATCCCA160.12052730696798493No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTCATCC160.12052730696798493No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC150.11299435028248588No Hit
AATCCTTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC150.11299435028248588No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA150.11299435028248588No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCCCCGCCAGGTC150.11299435028248588No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGGGTATCTAATCCCAGTCAT150.11299435028248588No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC140.10546139359698682No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCCACGGGGGGTATCTAATCCCAG140.10546139359698682No Hit
AATCCTATTAGAACAGGCTCCTCGTCGTCGGGGTATCTAATCCCAGTCAT140.10546139359698682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATT12450.0295.000033
ATCCTAT12650.0295.02
ATCCTAC108.371005E-4295.02
AATCCTA12800.0295.01
CCTACTA108.371005E-4295.04
CCTATTA12350.0295.04
CTATAGA108.371005E-4295.05
TCCTACT108.371005E-4295.03
ATTAGAA12250.0295.07
CCCATTA108.371005E-4295.04
CATTAGA108.371005E-4295.06
CCATCTA108.371005E-4295.04
CCATTAG108.371005E-4295.05
TCCATCT108.371005E-4295.03
CGCACGA157.080267E-6294.99997295
CTATTAC157.080267E-6294.999975
CTATTAG12300.0292.601625
TTAGAAC12400.0291.431468
TATTAGA12350.0291.4176
TAGAACA13300.0279.473669
Produced by FastQC (version 0.11.5)