FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-4-5-4-1-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-4-5-4-1-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10950
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT367833.58904109589041No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT9848.986301369863012No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG7717.041095890410959No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT5394.922374429223744No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC3753.4246575342465753No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2562.3378995433789953No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC2522.3013698630136985No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2352.146118721461187No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG1851.6894977168949772No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG1411.2876712328767124No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1401.2785388127853883No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1201.095890410958904No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1111.0136986301369864No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1060.9680365296803652No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCC1010.9223744292237444No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCT990.904109589041096No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG950.8675799086757991No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA920.8401826484018264No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT670.6118721461187215No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTAC540.4931506849315068No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT530.4840182648401826No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT500.45662100456621No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACC470.4292237442922374No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT420.3835616438356164No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT390.3561643835616438No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCC370.3378995433789954No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACC370.3378995433789954No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTACC350.31963470319634707No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG340.31050228310502287No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT330.3013698630136986No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTAC320.2922374429223744No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC310.2831050228310502No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCATTGGGGTATCTAA300.273972602739726No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT300.273972602739726No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTG290.2648401826484018No Hit
GAGACTTAGAACAGGCTCCTCTAGATCTCGTGGGGGTATCTAATCCCAGT280.2557077625570776No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA260.2374429223744292No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG250.228310502283105No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTAC250.228310502283105No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT250.228310502283105No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT240.2191780821917808No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC240.2191780821917808No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGGTATCTAATCCCAGT230.2100456621004566No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT220.2009132420091324No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT220.2009132420091324No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTAC210.1917808219178082No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT210.1917808219178082No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCAG210.1917808219178082No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT200.182648401826484No Hit
GAGACTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTACC190.1735159817351598No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT190.1735159817351598No Hit
GAGACTTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAG190.1735159817351598No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCC190.1735159817351598No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTAC180.16438356164383564No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTAC180.16438356164383564No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTGT170.15525114155251143No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT170.15525114155251143No Hit
GAGACTTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTGTA160.1461187214611872No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATCTA160.1461187214611872No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACCC160.1461187214611872No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTA160.1461187214611872No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA150.136986301369863No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT150.136986301369863No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT140.1278538812785388No Hit
GAGACTTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG140.1278538812785388No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAAT130.1187214611872146No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCC120.1095890410958904No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT110.1004566210045662No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT110.1004566210045662No Hit
GAGACTTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT110.1004566210045662No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTAC110.1004566210045662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTA10750.0295.02
GACTTAG10650.0295.03
GAGACTT10800.0295.01
ACTTAGA10700.0293.62154
CTTAGAA10650.0293.615025
TTAGAAC10700.0290.864476
GAACAGG10750.0286.767439
TAGAACA10950.0285.570777
AGAACAG11000.0280.258
GACTTAA150.0028114356196.666673
GAACAAG200.006647462147.59
ACGAAGA700.003245594263.214283295
ACTGCCA452.5465852E-1159.00000435-39
GGGTTTT900.059.00000450-54
TCAAGGT452.5465852E-1159.000004105-109
TACGGCT950.059.000004115-119
CACTGCC452.5465852E-1159.00000435-39
GTTTACG950.059.000004110-114
TCTCGTG900.059.00000425-29
CCTTTGG950.059.00000445-49
Produced by FastQC (version 0.11.5)