FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-39-6-4-8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-39-6-4-8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13051
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC355627.24695425637882No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC227317.41628993946824No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC6555.018772507853804No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC5634.313845682323194No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC5544.244885449390852No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG5023.846448548003984No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC4613.532296375756647No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC4483.4326871504099308No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC4473.425024902306337No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTT3953.02658800091947No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC3182.436594896942763No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCA2662.0381579955558964No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTATCCATCGTTTACGGCTAGGACT2401.8389395448624626No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGATCTAAAGTACCGCCAAGTC1891.4481648915791894No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGAAGGGGTATCTAATCCCAGTGTC1721.3179066738180982No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1671.2795954333001303No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGGTCTGAGGCACCGCCAAGT1571.2029729522641943No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTC1220.9347942686384185No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAAT1010.773887058462953No Hit
TCTCAATCTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG740.5670063596659259No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGT410.3141521722473374No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTCAA390.2988276760401502No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC150.11493372155390391No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC140.10727147345031034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAG12650.0295.05
CAATCTA12700.0295.04
CTCATCT108.369551E-4295.02
TCTCATC108.369551E-4295.01
TCTCAAT12900.0293.85661
CTCAATC12800.0293.847662
TCAATCT12750.0293.84313
TCTAGAA12700.0291.515757
ATCTAGA12800.0290.390626
CTAGAAC12850.0286.9658
TAGAACA13650.0270.14659
CATCTAG150.0028176361196.666674
TCATCTA150.0028176361196.666673
ACAGCAA700.003259716363.214283295
TGGTTGT150.0043620459.00000490-94
GGTTGTA150.0043620459.00000490-94
TTCATAA150.0043620459.000004220-224
ACTCGGT303.2936077E-759.000004205-209
AATCTCG150.0043620459.000004205-209
AATCCAG150.0043620459.000004140-144
Produced by FastQC (version 0.11.5)